The C/EBPβ LIP isoform rescues loss of C/EBPβ function in the mouse

Scientific Reports
Valérie BégayAchim Leutz

Abstract

The transcription factor C/EBPβ regulates hematopoiesis, bone, liver, fat, and skin homeostasis, and female reproduction. C/EBPβ protein expression from its single transcript occurs by alternative in-frame translation initiation at consecutive start sites to generate three isoforms, two long (LAP*, LAP) and one truncated (LIP), with the same C-terminal bZip dimerization domain. The long C/EBPβ isoforms are considered gene activators, whereas the LIP isoform reportedly acts as a dominant-negative repressor. Here, we tested the putative repressor functions of the C/EBPβ LIP isoform in mice by comparing monoallelic WT or LIP knockin mice with Cebpb knockout mice, in combination with monoallelic Cebpa mice. The C/EBPβ LIP isoform was sufficient to function in coordination with C/EBPα in murine development, adipose tissue and sebocyte differentiation, and female fertility. Thus, the C/EBPβ LIP isoform likely has more physiological functions than its currently known role as a dominant-negative inhibitor, which are more complex than anticipated.

References

Aug 25, 1995·Science·N D WangG J Darlington
Sep 26, 1997·Genes & Development·E SterneckP F Johnson
Jun 15, 2000·The American Journal of Pathology·J P SundbergK Stenn
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q Q Tang, M D Lane
Aug 23, 2002·Molecular and Cellular Biology·Charles VinsonMaria Bonovich
Jan 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Qi-Qun TangM Daniel Lane
Dec 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jiang-Wen ZhangM Daniel Lane
Oct 29, 2004·Molecular and Cellular Biology·Valérie BégayAchim Leutz
Jun 6, 2006·Nature Immunology·Hideyo HiraiDaniel G Tenen
Jul 20, 2007·Obesity·Yasuyo AriyamaMasatomo Mori
Nov 5, 2008·Genes & Development·Martina I LefterovaMitchell A Lazar
Dec 6, 2008·Carcinogenesis·Jennifer StaigerPeter F Johnson
Jul 27, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Claus Nerlov
Feb 16, 2011·Journal of Molecular Endocrinology·Raghunath ChatterjeeCharles Vinson
Sep 29, 2011·Nature Reviews. Molecular Cell Biology·Ana G Cristancho, Mitchell A Lazar
May 27, 2014·Cell Reports·Rasmus SiersbækSusanne Mandrup
Nov 18, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Valérie BégayAchim Leutz
Jun 27, 2015·EMBO Reports·Laura M ZidekCornelis F Calkhoven

❮ Previous
Next ❯

Citations

May 28, 2021·Science Advances·Sophie KasparAleksandra Trifunovic
Nov 19, 2021·Journal of Animal Physiology and Animal Nutrition·Mengli YangYun Ma
Dec 28, 2021·Adipocyte·Alyssa ThunenJohn E Shively
Oct 31, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Leonardo GatticchiRita Roberti

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

Prism
GraphPad
Zeiss AxioVision

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved