The cel4 gene of Agaricus bisporus encodes a beta-mannanase

Applied and Environmental Microbiology
C M TangC F Thurston

Abstract

Mannases have industrial uses in food and pulp industries, and their regulation may influence development of the mushrooms of commercially important basidiomycetes. We expressed an Agaricus bisporus cel4 cDNA, which encodes a mannanase, in Saccharomyces cerevisiae and Pichia pastoris. CEL4 had no detectable activity on cellulose or xylan. This gene is the first isolated from this economically important fungus to encode a mannanase. P. pastoris secreted about three times more CEL4 than S. cerevisiae. The removal of the cellulose-binding domain of CEL4 lowered the secreted specific activity by P. pastoris by approximately 97%. The genomic sequence of cel4 was isolated by screening a cosmid library of A. bisporus C54-carb8. The open reading frame was interrupted by 12 introns. The level of extracellular CEL4 increases dramatically at the postharvest stage in compost extracts of A. bisporus fruiting cultures. In laboratory liquid cultures of A. bisporus, the activity of CEL4 detected in the culture filtrate reached a maximum after 21 days. The levels of CEL4 broadly mirrored the levels of enzyme activity. In the Solka floc-bound mycelium, CEL4 protein showed a maximum after 2 to 3 weeks of culture and then declined. Changes in CEL4...Continue Reading

References

Aug 1, 1991·Bio/technology·G A BowdenG Georgiou
Sep 1, 1988·Trends in Genetics : TIG·T Humphrey, N J Proudfoot
Aug 1, 1993·Bio/technology·J M CreggW C Raschke
Apr 1, 1994·Molecular Microbiology·E YagüeC F Thurston
Jun 1, 1993·Journal of General Microbiology·C R PerryC F Thurston
Dec 10, 1998·Nucleic Acids Research·R GuptaJ E Hansen
Mar 1, 1994·Applied and Environmental Microbiology·A M BonnenA B Orth
Nov 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M G LoftusT J Elliott

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Citations

Feb 7, 2015·Biotechnology Letters·Ankita Shrivastava BhattacharyaBrett I Pletschke
Nov 28, 2014·Microbiology and Molecular Biology Reviews : MMBR·Johanna RytiojaMiia R Mäkelä
Dec 21, 2016·Biological Reviews of the Cambridge Philosophical Society·Simon LadevèzeGabrielle Potocki-Véronèse
Dec 19, 2007·Critical Reviews in Biotechnology·Samriti Dhawan, Jagdeep Kaur
Apr 20, 2006·Journal of Biotechnology·Shuuji OotsukaTakao Ojima

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