The cell biologist's guide to super-resolution microscopy.

Journal of Cell Science
Guillaume JacquemetC. Leterrier

Abstract

Fluorescence microscopy has become a ubiquitous method to observe the location of specific molecular components within cells. However, the resolution of light microscopy is limited by the laws of diffraction to a few hundred nanometers, blurring most cellular details. Over the last two decades, several techniques - grouped under the 'super-resolution microscopy' moniker - have been designed to bypass this limitation, revealing the cellular organization down to the nanoscale. The number and variety of these techniques have steadily increased, to the point that it has become difficult for cell biologists and seasoned microscopists alike to identify the specific technique best suited to their needs. Available techniques include image processing strategies that generate super-resolved images, optical imaging schemes that overcome the diffraction limit and sample manipulations that expand the size of the biological sample. In this Cell Science at a Glance article and the accompanying poster, we provide key pointers to help users navigate through the various super-resolution methods by briefly summarizing the principles behind each technique, highlighting both critical strengths and weaknesses, as well as providing example images.

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Citations

Jan 16, 2021·Current Opinion in Neurobiology·Christophe Leterrier
Oct 17, 2020·The Journal of Biological Chemistry·David L ProleNick S Jones
Jan 18, 2021·The Journal of Biological Chemistry·David L ProleNick S Jones
Mar 16, 2021·Frontiers in Molecular Neuroscience·Benjamin T Goult
Apr 2, 2021·Plant Physiology·Zhiqi LiuLiwen Jiang
Apr 17, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Shawn Yoshida, Lydia Kisley
Apr 27, 2021·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Hai GongMark A A Neil
May 1, 2021·International Journal of Molecular Sciences·Ewa BączyńskaBłażej Ruszczycki
Jul 3, 2021·International Journal of Molecular Sciences·Lidia V Putlyaeva, Konstantin A Lukyanov
Jul 20, 2021·Disease Models & Mechanisms·Julija Hmeljak, Esperanza Agullo-Pascual
Aug 8, 2021·Cells·Joshua J A Poole, Leila B Mostaço-Guidolin
Aug 27, 2021·Current Protocols·Jessica Valli, Jeremy Sanderson
Aug 28, 2021·International Journal of Molecular Sciences·Thomas K SmyllaArmin Huber

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