The cell birth marker BrdU does not affect recruitment of subsequent cell divisions in the adult avian brain

BioMed Research International
Anat CattanAnat Barnea

Abstract

BrdU is commonly used to quantify neurogenesis but also causes mutation and has mitogenic, transcriptional, and translational effects. In mammalian studies, attention had been given to its dosage, but in birds such examination was not conducted. Our previous study suggested that BrdU might affect subsequent cell divisions and neuronal recruitment in the brain. Furthermore, this effect seemed to increase with time from treatment. Accordingly, we examined whether BrdU might alter neurogenesis in the adult avian brain. We compared recruitment of [(3)H]-thymidine(+) neurons in brains of zebra finches (Taeniopygia guttata) when no BrdU was involved and when BrdU was given 1 or 3 months prior to [(3)H]-thymidine. In nidopallium caudale, HVC, and hippocampus, no differences were found between groups in densities and percentages of [(3)H]-thymidine(+) neurons. The number of silver grains per [(3)H]-thymidine(+) neuronal nucleus and their distribution were similar across groups. Additionally, time did not affect the results. The results indicate that the commonly used dosage of BrdU in birds has no long-term effects on subsequent cell divisions and neuronal recruitment. This conclusion is also important in neuronal replacement experimen...Continue Reading

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Citations

Mar 21, 2019·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Pilani NkomozepiAmadi O Ihunwo
Jul 28, 2018·Scientific Reports·Tatyana PoznerAnat Barnea
Oct 30, 2020·Biology·Lubica KubikovaKristina Lukacova

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

Neurolucida
STATISTICA
SPSS

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