The cell-penetrating peptide domain from human heparin-binding epidermal growth factor-like growth factor (HB-EGF) has anti-inflammatory activity in vitro and in vivo

Biochemical and Biophysical Research Communications
Jue Yeon LeeChong-Pyoung Chung

Abstract

A heparin-binding peptide (HBP) sequence from human heparin-binding epidermal growth factor-like growth factor (HB-EGF) was identified and was shown to exhibit cell penetration activity. This cell penetration induced an anti-inflammatory reaction in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. HBP penetrated the cell membrane during the 10 min treatment and reduced the LPS-induced production of nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cytokines (TNF-α and IL-6) in a concentration-dependent manner. Additionally, HBP inhibited the LPS-induced upregulation of cytokines, including TNF-α and IL-6, and decreased the interstitial infiltration of polymorphonuclear leukocytes in a lung inflammation model. HBP inhibited NF-κB-dependent inflammatory responses by directly blocking the phosphorylation and degradation of IκBα and by subsequently inhibiting the nuclear translocation of the p65 subunit of NF-κB. Taken together, this novel HBP may be potentially useful candidate for anti-inflammatory treatments and can be combined with other drugs of interest to transport attached molecules into cells.

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Citations

May 8, 2013·Therapeutic Delivery·Luís VasconcelosUlo Langel
Oct 8, 2016·Biotechnology Letters·Bing LinJian Zhao
Oct 16, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Zhao LuoFu-Jun Wang
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Mar 7, 2020·Expert Opinion on Drug Delivery·Larissa Gomes Dos Reis, Daniela Traini
Apr 6, 2021·Journal of Biomedical Materials Research. Part a·Jue-Yeon LeeYoon Jeong Park

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