PMID: 9537359Apr 16, 1998Paper

The cell wall-anchored Streptomyces reticuli avicel-binding protein (AbpS) and its gene

Journal of Bacteriology
S WalterH Schrempf

Abstract

Streptomyces reticuli produces a 35-kDa cellulose-binding protein (AbpS) which interacts strongly with crystalline forms of cellulose (Avicel, bacterial microcrystalline cellulose, and tunicin cellulose); other polysaccharides are recognized on weakly (chitin and Valonia cellulose) or not at all (xylan, starch, and agar). The protein could be purified to homogeneity due to its affinity to Avicel. After we sequenced internal peptides, the corresponding gene was identified by reverse genetics. In vivo labelling experiments with fluorescein isothiocyanate (FITC), FITC-labelled secondary antibodies, or proteinase K treatment revealed that the anchored AbpS protrudes from the surfaces of the hyphae. When we investigated the hydrophobicity of the deduced AbpS, one putative transmembrane segment was predicted at the C terminus. By analysis of the secondary structure, a large centrally located alpha-helix which has weak homology to the tropomyosin protein family was found. Physiological studies showed that AbpS is synthesized during the late logarithmic phase, independently of the carbon source.

References

Jan 1, 1992·Annual Review of Genetics·J S Parkinson, E C Kofoid
Jul 1, 1987·Analytical Biochemistry·W H SchwarzW L Staudenbauer
Jul 1, 1986·Journal of Bacteriology·R BelasM Silverman
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G N PhillipsV A Fischetti
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Aug 15, 1994·FEMS Microbiology Letters·H F Jenkinson
Jun 4, 1993·Cell·J S Parkinson
Jan 1, 1994·FEMS Microbiology Reviews·P Béguin, J P Aubert
Jan 1, 1996·Annual Review of Microbiology·G Deckers-Hebestreit, K Altendorf
Oct 1, 1989·Applied and Environmental Microbiology·G WachingerH Schrempf
Oct 1, 1993·Applied and Environmental Microbiology·P Vandevivere, D L Kirchman
Mar 1, 1996·Applied and Environmental Microbiology·S Walter, H Schrempf

❮ Previous
Next ❯

Citations

May 9, 2003·The Journal of Biological Chemistry·Stefan Walter, Hildgund Schrempf
Jan 22, 2010·FEMS Microbiology Reviews·Keith F ChaterHildgund Schrempf
Oct 12, 2021·FEMS Microbiology Reviews·Wiem AbidiPetya Violinova Krasteva

❮ Previous
Next ❯