DOI: 10.1101/455782Nov 6, 2018Paper

The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis.

BioRxiv : the Preprint Server for Biology
Joseph Franics McKennaJohn Runions


Plant plasma-membrane (PM) proteins are involved in several vital processes, such as detection of pathogens, solute transport and cellular signalling. Recent models suggest that for these proteins to function effectively there needs to be structure within the PM allowing, for example, proteins in the same signalling cascade to be spatially organized. Here we demonstrate that several proteins with divergent functions are located in clusters of differing size in the membrane using sub-diffraction-limited Airyscan confocal microscopy. In addition, single particle tracking reveals that these proteins move at different rates within the membrane. We show that the actin and microtubule cytoskeletons appear to significantly regulate the mobility of one of these proteins (the pathogen receptor FLS2) and we further demonstrate that the cell wall is critical for the regulation of cluster size by affecting single particle dynamics of two proteins with key roles in morphogenesis (PIN3) and pathogen perception (FLS2). We propose a model in which the cell wall and cytoskeleton are pivotal for differentially regulating protein cluster size and dynamics thereby contributing to the formation and functionality of membrane nanodomains.

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Current Opinion in Plant Biology
Joseph Franics McKennaJohn Runions
Seminars in Cell & Developmental Biology
Elizabeth GrangerPhilip Woodman
Journal of Cellular Biochemistry
Lakshmi BhaskarRaghava Varman Thampan
© 2021 Meta ULC. All rights reserved