The cellular actions of interleukin-11 on bone resorption in vitro

Endocrinology
P A HillM C Meikle

Abstract

The pleiotropic cytokine interleukin-11 (IL-11) stimulates osteoclast formation in vitro, but it is not known whether it influences other steps in the bone-resorptive cascade. Using a variety of in vitro model systems for studying bone resorption we have investigated the effects of IL-11 on 1) osteoclast formation, fusion, migration, and activity; and 2) osteoblast-mediated osteoid degradation. The involvement of matrix metalloproteinases (MMPs) and products of arachidonic acid metabolism in IL-11-mediated resorption were also assessed. We first examined the bone-resorptive effects of IL-11 by assessing 45Ca release from neonatal mouse calvarial bones. IL-11 dose-dependently stimulated bone resorption with an EC50 of 10(-10) M. The kinetics of IL-11-mediated 45Ca release demonstrated that it was without effect for the first 48 h of culture, but by 96 h, it stimulated 45Ca release to the same level as that produced by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] (a hormone that stimulates osteoclast formation and activity). IL-11 also produced a dose-dependent increase in osteoblast-mediated type I collagen degradation with a maximum of 58.0 +/- 6.2% at 5 x 10(-9) M; this effect of IL-11 was less than that produced by 1,25-(OH)2D3 (7...Continue Reading

Citations

Dec 7, 2010·The Journal of Biological Chemistry·Vishnu C RamaniRalph D Sanderson
Mar 6, 2013·Biochemia Medica·Janja ZupanJanja Marc
Nov 13, 2004·Reproductive Biology and Endocrinology : RB&E·Christine A WhiteLois A Salamonsen
May 3, 2008·Endocrine Reviews·Joseph LorenzoYongwon Choi
Oct 28, 2006·Molecular Endocrinology·Gregory A ClinesTheresa A Guise
Jan 5, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K SakaiY Iwamoto
Oct 12, 2000·Cytokine·D Heymann, A V Rousselle
Mar 19, 2014·Journal of Bone and Mineral Metabolism·Hiroyoshi MatsumuraYorimasa Ogata
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·V J Kingsmill
Mar 5, 2004·International Journal of Cancer. Journal International Du Cancer·Hayley MorganPeter A Hill
Jun 10, 2006·Current Opinion in Rheumatology·Sun-Kyeong Lee, Joseph Lorenzo
Aug 10, 2000·Breast Cancer Research and Treatment·M LacroixJ J Body
Feb 5, 2008·Nutrition Reviews·Gregory R Mundy
Jun 9, 2001·Experimental Biology and Medicine·B A WatkinsM F Seifert
Jul 12, 2011·American Journal of Human Genetics·Pekka NieminenIrma Thesleff

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