The cellular basis for the defect in haemopoiesis in flexed-tailed mice. III. Restriction of the defect to erythropoietic progenitors capable of transient colony formation in vivo

British Journal of Haematology
C J GregoryJ E Till

Abstract

Three assays for erythropoietic progenitor cells have been applied to mice of genotype f/f and to nearly congenic +/+ controls. When f/f mice were tested for their ability to generate transient endogenous erythroid spleen colonies 4-6 days after 800 rads and 10 units of erythropoietin, the numbers of such colonies detected were greatly reduced, although normal numbers of spleen colonies appeared at later times (9-12 days) postirradiation. In contrast, cells capable of erythropoietic colony formation in culture (CFU-E) were present within the normal range in both f/f spleen and marrow and their sensitivity to erythropoietin in culture was the same as that found previously for CFU-E in the marrow and spleen of +/+ mice. Transfusion-induced plethora reduced the number of CFU-E in marrow to a similar extent in both f/f and +/+ mice; likewise, subsequent administration of 10 units of erythropoietin induced a rapid return in the number of marrow CFU-E in both genotypes. In the spleen, CFU-E numbers were approximately three-fold lower in f/f mice in each group. These results support the view that the 5 day assay for transient endogenous spleen colonies detects cells (TE-CFU) that are different from both CFU-E and pluripotent stem cell...Continue Reading

References

Jul 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J R StephensonM M Shreeve
Dec 31, 1974·Biochimica Et Biophysica Acta·E A McCullochJ E Till
Jun 1, 1973·Journal of Cellular Physiology·C J GregoryJ E Till
Oct 1, 1969·Journal of Cellular Physiology·R G WortonJ E Till
Mar 1, 1969·Genetics·D L ColemanE Y Levin
Jun 1, 1968·Journal of Cellular Physiology·M BennettD Metcalf
Mar 31, 1964·Annals of the New York Academy of Sciences·J E TILL, E A MCCULLOCH
May 15, 1964·Science·E A MCCULLOCHJ E TILL
Dec 1, 1964·Journal of Cellular Physiology·K I ALTMAN, E S RUSSELL

❮ Previous
Next ❯

Citations

Feb 1, 1977·Journal of Cellular Physiology·G Van Zant, E Goldwasser
Mar 12, 2020·Cells·Robert F PaulsonYuanting Chen
Sep 12, 2020·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Yung HwangMerav Socolovsky
Jul 1, 1976·British Journal of Haematology·R J Cole, T Regan
Dec 7, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Shailaja HegdeRobert F Paulson
Feb 22, 2018·Nature·Betsabeh Khoramian TusiMerav Socolovsky
Dec 14, 2004·Blood·Laurie E LenoxRobert F Paulson
Apr 1, 1979·Journal of Cellular Physiology·W Wiktor-JedrzejczakK W Sell
Dec 21, 2018·The Journal of Physiological Sciences : JPS·Ei Ei MonKazuhito Tomizawa
Oct 19, 2007·Journal of Virology·Aparna SubramanianRobert F Paulson
Nov 1, 2008·Experimental Hematology·Lisa J McReynoldsTodd Evans
Apr 21, 2009·Experimental Hematology·Laurie E LenoxRobert F Paulson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.