The cellular internalization of liposome encapsulated protoporphyrin IX by HeLa cells

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
Magdalena PrzybyloM Langner

Abstract

The proper lipid composition of liposomes designed to carry drugs determines their surface properties ensuring their accumulation within selected tissue. The electrostatic potential and surface topology of liposomes affect the internalization by single cells. The high-resolution imaging of cancer cells and the distribution of protoporphyrin-loaded liposomes within the cytoplasm and its dependence on the liposome surface properties are presented. In the paper, HeLa cells were used to investigate the uptake of porphyrin-loaded liposomes and liposomes alone by means of confocal and differential interference contrast microscopies. The effect of liposomes surface electrostatic potential and surface topology on their intracellular distribution was evaluated. The time evolution of the intracellular distribution of liposomes labelled with Rhodamine-PE was examined on HeLa cells. These studies allow for the identification of the liposome lipid composition so the efficient delivery of the active substance to cancer cells will be achieved. The obtained results showed that neutral PC-liposomes are the most efficiently internalized by HeLa cells. Moreover, results showed that properties of liposomes affect not only the internalization effic...Continue Reading

References

Jan 10, 1992·Biochimica Et Biophysica Acta·A Gabizon, D Papahadjopoulos
Apr 1, 1996·The Journal of Pharmacy and Pharmacology·A N AnigboguB W Barry
Jun 2, 2001·Journal of Photochemistry and Photobiology. B, Biology·X DamoiseauM Hoebeke
Mar 19, 2002·Biophysical Chemistry·Teresa KralMartin Hof
Jan 5, 2005·Journal of Photochemistry and Photobiology. B, Biology·P G Calzavara-PintonR Sala
Jul 26, 2005·Cytotherapy·R Sidi Boumédine, D C Roy
Oct 7, 2005·Drug Development and Industrial Pharmacy·Toshiyuki KosobeNorimichi Kawashima
Oct 26, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Julia BuchholzHeinrich Walt
Nov 15, 2006·Cellular & Molecular Biology Letters·Katarzyna StebelskaAleksander F Sikorski
Jun 6, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Tobias KiesslichFrieder Berr
Dec 22, 2007·Advanced Drug Delivery Reviews·Panayiotis P ConstantinidesRobert Shorr
Mar 15, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Jennifer S HongSrinivasa R Raghavan
Oct 22, 2008·Advanced Drug Delivery Reviews·Dev Kumar ChatterjeeYong Zhang
Dec 23, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Henri-Pierre LassalleLina Bezdetnaya
Jun 6, 2009·Pigment Cell & Melanoma Research·Melissa A TranGavin P Robertson
Oct 14, 2009·Progress in Histochemistry and Cytochemistry·Vadim Zinchuk, Olga Grossenbacher-Zinchuk
Aug 18, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Pierre-Henri GuelluyMaryse Hoebeke
Nov 3, 2010·The Journal of Pharmacy and Pharmacology·Heike Bunjes
Oct 12, 2013·CA: a Cancer Journal for Clinicians·Avnesh S Thakor, Sanjiv S Gambhir

❮ Previous
Next ❯

Citations

Jun 21, 2018·Physical Chemistry Chemical Physics : PCCP·Maria Eugenia Fortes BrolloMaría Del Puerto Morales
Sep 11, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Lalit ChudalWei Chen
Jan 7, 2020·ACS Applied Materials & Interfaces·Maria Eugenia Fortes BrolloMaría Del Puerto Morales

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.