The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres

Nature Cell Biology
Masahiro OkadaTatsuo Fukagawa

Abstract

In vertebrates, centromeres lack defined sequences and are thought to be propagated by epigenetic mechanisms involving the incorporation of specialized nucleosomes containing the histone H3 variant centromere protein (CENP)-A. However, the precise mechanisms that target CENP-A to centromeres remain poorly understood. Here, we isolated a multi-subunit complex, which includes the established inner kinetochore components CENP-H and CENP-I, and nine other proteins, from both human and chicken cells. Our analysis of these proteins demonstrates that the CENP-H-I complex can be divided into three functional sub-complexes, each of which is required for faithful chromosome segregation. Interestingly, newly expressed CENP-A is not efficiently incorporated into centromeres in knockout mutants of a subclass of CENP-H-I complex proteins, indicating that the CENP-H-I complex may function, in part, as a marker directing CENP-A deposition to centromeres.

References

Apr 1, 1987·The Journal of Cell Biology·D K PalmerR L Margolis
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·E V HowmanK H Choo
Nov 22, 2000·The Journal of Cell Biology·R D ShelbyK F Sullivan
Jun 13, 2001·The Journal of Cell Biology·K OegemaA A Hyman
Apr 24, 2002·Developmental Cell·Ai NishihashiTatsuo Fukagawa
Oct 29, 2003·The Journal of Cell Biology·Stefan WestermannGeorjana Barnes
Nov 25, 2003·Genes & Development·Peter De WulfPeter K Sorger
May 4, 2004·Experimental Cell Research·Tatsuo Fukagawa
Jan 13, 2005·Science's STKE : Signal Transduction Knowledge Environment·Iain M Cheeseman, Arshad Desai
Feb 17, 2005·Molecular and Cellular Biology·Yoshikazu MikamiTatsuo Fukagawa
May 5, 2005·Molecular and Cellular Biology·Vinciane RégnierWilliam Brown
Aug 5, 2005·The EMBO Journal·Xingkun LiuXiangwei He
Nov 17, 2005·Molecular and Cellular Biology·Yukinori MinoshimaTatsuo Fukagawa
Apr 20, 2006·Nature Cell Biology·Daniel R FoltzDon W Cleveland

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Citations

Mar 26, 2010·Cellular and Molecular Life Sciences : CMLS·Bruce F McEwen, Yimin Dong
Jun 18, 2009·European Biophysics Journal : EBJ·D HellwigStephan Diekmann
Sep 24, 2010·Chromosoma·Bernardo Orr, Claudio E Sunkel
Jul 14, 2011·Chromosoma·Marinela Perpelescu, Tatsuo Fukagawa
Jun 30, 2010·Molecular Genetics and Genomics : MGG·Gunjan D MehtaSantanu Kumar Ghosh
Feb 12, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·John Tooley, P Todd Stukenberg
Jun 27, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Tetsuya Hori, Tatsuo Fukagawa
Jun 29, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Luis P ValenteLars E T Jansen
Apr 9, 2013·Progress in Biophysics and Molecular Biology·Sergej TschernyschkowBashar Ibrahim
Mar 14, 2013·Trends in Cell Biology·Stefan Westermann, Alexander Schleiffer
Apr 20, 2013·Trends in Biochemical Sciences·Luying JiaHongtao Yu
May 13, 2006·Nature Cell Biology·Barbara MelloneGary H Karpen
Apr 20, 2006·Nature Cell Biology·Daniel R FoltzDon W Cleveland
Jun 23, 2009·Nature Cell Biology·Christopher W CarrollAaron F Straight
Mar 17, 2010·Nature Cell Biology·Ana C AmaroPatrick Meraldi
May 9, 2012·Nature Cell Biology·Alexander SchleifferStefan Westermann
Jul 23, 2013·Nature Cell Biology·Daniele FachinettiDon W Cleveland
Nov 13, 2008·Nature Reviews. Genetics·Robin C Allshire, Gary H Karpen
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, Edward D Salmon
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·Iain M Cheeseman, Arshad Desai
Apr 22, 2011·Nature Reviews. Molecular Cell Biology·Jolien S Verdaasdonk, Kerry Bloom
Jul 15, 2009·Nature Structural & Molecular Biology·Sang-Moon YunKyung S Lee
May 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Susana Abreu RibeiroWilliam C Earnshaw
Apr 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jung-Eun ParkKyung S Lee
Jul 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Maryam SaneiAndreas Houben
Dec 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marlyn GonzalezFei Li
Nov 17, 2009·The Journal of Biological Chemistry·Haitong HouSongtao Jia
Feb 20, 2010·The Journal of Biological Chemistry·Akihisa OsakabeHitoshi Kurumizaka
Nov 9, 2010·The Journal of Biological Chemistry·Shasha HuaXuebiao Yao
Apr 2, 2011·The Journal of Biological Chemistry·Young H KangKyung S Lee
Mar 30, 2007·Molecular Biology of the Cell·Mi-Sun KwonTatsuo Fukagawa
Dec 21, 2007·Molecular Biology of the Cell·Tetsuya HoriTatsuo Fukagawa
Nov 30, 2007·Molecular Biology of the Cell·Iain M CheesemanArshad Desai
Jul 25, 2009·Molecular Biology of the Cell·Masahiro OkadaTatsuo Fukagawa
Jul 31, 2009·Molecular Biology of the Cell·Kirstin J MilksAaron F Straight
Aug 7, 2009·Molecular Biology of the Cell·Stefano CardinaleWilliam C Earnshaw
Jan 18, 2013·Nucleic Acids Research·Hiroaki TachiwanaHitoshi Kurumizaka

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