The central domain of Escherichia coli TyrR is responsible for hexamerization associated with tyrosine-mediated repression of gene expression.

The Journal of Biological Chemistry
Mathew P DixonBarrie E Davidson

Abstract

TyrR from Escherichia coli regulates the expression of genes for aromatic amino acid uptake and biosynthesis. Its central ATP-hydrolyzing domain is similar to conserved domains of bacterial regulatory proteins that interact with RNA polymerase holoenzyme associated with the alternative sigma factor, sigma(54). It is also related to the common module of the AAA+ superfamily of proteins that is involved in a wide range of cellular activities. We expressed and purified two TyrR central domain polypeptides. The fragment comprising residues 188-467, called TyrR-(188-467), was soluble and stable, in contrast to that corresponding to the conserved core from residues 193 to 433. TyrR-(188-467) bound ATP and rhodamine-ATP with association constants 2- to 5-fold lower than TyrR and hydrolyzed ATP at five times the rate of TyrR. In contrast to TyrR, which is predominantly dimeric at protein concentrations less than 10 microm in the absence of ligands, or in the presence of ATP or tyrosine alone, TyrR-(188-467) is a monomer, even at high protein concentrations. Tyrosine in the presence of ATP or ATPgammaS promotes the oligomerization of TyrR-(188-467) to a hexamer. Tyrosine-dependent repression of gene transcription by TyrR therefore depen...Continue Reading

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Citations

Aug 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·J Pittard
Dec 23, 2004·Molecular Microbiology·James PittardJi Yang
Oct 22, 2008·Journal of Bacteriology·Takashi KoyanagiHidehiko Kumagai
Sep 2, 2003·Journal of Bacteriology·Zehra Tüzün Güvener, Linda L McCarter
Mar 6, 2003·Journal of Bacteriology·David J Studholme, Ray Dixon
Jan 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Bettina HartliebStephan Becker
Dec 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Bruno Y MatsuyamaMarcos V A S Navarro
Aug 15, 2003·The Journal of Biological Chemistry·Belinda J WestmanJoel P Mackay
Aug 20, 2015·Interdisciplinary Sciences, Computational Life Sciences·Utkarsh Raj, Pritish Kumar Varadwaj
Feb 11, 2015·Interdisciplinary Sciences, Computational Life Sciences·Utkarsh Raj, Pritish Kumar Varadwaj
Sep 1, 2008·EcoSal Plus·James Pittard, Ji Yang

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