The Centriolar Adjunct⁻Appearance and Disassembly in Spermiogenesis and the Potential Impact on Fertility

Cells
Anastasiia S GaraninaRustem E Uzbekov

Abstract

During spermiogenesis, the proximal centriole forms a special microtubular structure: the centriolar adjunct. This structure appears at the spermatid stage, which is characterized by a condensed chromatin nucleus. We showed that the centriolar adjunct disappears completely in mature porcine spermatozoa. In humans, the centriolar adjunct remnants are present in a fraction of mature spermatids. For the first time, the structure of the centriolar adjunct in the cell, and its consequent impact on fertility, were examined. Ultrastructural analysis using transmission electron microscopy was performed on near 2000 spermatozoa per person, in two patients with idiopathic male sterility (IMS) and five healthy fertile donors. We measured the average length of the "proximal centriole + centriolar adjunct" complex in sections, where it had parallel orientation in the section plane, and found that it was significantly longer in the spermatozoa of IMS patients than in the spermatozoa of healthy donors. This difference was independent of chromatin condensation deficiency, which was also observed in the spermatozoa of IMS patients. We suggest that zygote arrest may be related to an incompletely disassembled centriolar adjunct in a mature sperma...Continue Reading

References

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Jun 9, 2018·Nature Communications·Emily L FishmanTomer Avidor-Reiss
Jul 13, 2018·Biology Open·Rustem UzbekovChristophe Bressac

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Citations

Sep 30, 2020·Cells·Rustem E Uzbekov, Tomer Avidor-Reiss
Oct 22, 2019·Frontiers in Cell and Developmental Biology·Tomer Avidor-ReissPuneet Sindhwani
Jan 19, 2021·Seminars in Cell & Developmental Biology·Swadhin Chandra Jana
Dec 18, 2020·Cells·Maria Giovanna RiparbelliGiuliano Callaini
Mar 4, 2021·Scientific Reports·Giulia CollodelElena Moretti
Aug 19, 2020·Molecular and Cellular Endocrinology·Tomer Avidor-ReissEmily Lillian Fishman

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Methods Mentioned

BETA
transmission electron microscopy

Software Mentioned

Digital Micrograph
Excel
Adobe Photoshop
Photoshop
IMOD
IMOD ( Image MODel )

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