The Cf-9 disease resistance protein is present in an approximately 420-kilodalton heteromultimeric membrane-associated complex at one molecule per complex

The Plant Cell
Susana RivasJonathan D G Jones

Abstract

The tomato Cf-9 gene confers race-specific resistance to the fungal pathogen Cladosporium fulvum expressing the corresponding avirulence gene Avr9. In tobacco, Cf-9 confers a hypersensitive response to the Avr9 peptide. To investigate Cf-9 protein function in initiating defense signaling, we engineered a functional C-terminal fusion of the Cf-9 gene with the TAP (Tandem Affinity Purification) tag. In addition, we established a transient expression assay in Nicotiana benthamiana leaves for the production of functional Cf-9:myc and Cf-9:TAP. Transiently expressed Cf-9:myc and Cf-9:TAP proteins induced an Avr9-dependent hypersensitive response, consistent with previous results with stably transformed tobacco plants and derived cell suspension cultures expressing c-myc-tagged Cf-9. Gel filtration of microsomal fractions solubilized with octylglucoside revealed that the Cf-9 protein, either as c-myc or TAP fusions, migrated at a molecular mass of 350 to 475 kD. By using blue native gel electrophoresis, the molecular size was confirmed to be approximately 420 kD. Our results suggest that only one Cf-9 protein molecule is present in the Cf-9 complex and that Cf-9 is part of a membrane complex consisting of an additional glycoprotein p...Continue Reading

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Citations

Mar 23, 2011·Biotechnology Letters·Yifeng Li
May 7, 2003·Current Opinion in Biotechnology·Kim E Hammond-Kosack, Jane E Parker
Aug 28, 2003·Microbes and Infection·Paul Muskett, Jane Parker
Aug 17, 2002·Current Opinion in Plant Biology·Seiji Takayama, Youji Sakagami
Jul 1, 2002·Molecular Plant Pathology·Susana Rivas, Colwyn M Thomas
Aug 1, 2002·The Plant Journal : for Cell and Molecular Biology·Susana RivasJonathan D G Jones
Jul 22, 2004·Plant Physiology·Iris TzafrirDavid Meinke
Feb 22, 2005·The Plant Cell·Renier A L van der HoornJonathan D G Jones
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