The cGMP/protein kinase G pathway contributes to dihydropyridine-sensitive calcium response and cytokine production in TH2 lymphocytes

The Journal of Biological Chemistry
Bruno GomesLucette Pelletier

Abstract

Th2 lymphocytes differ from other CD4+ T lymphocytes not only by their effector tasks but also by their T cell receptor (TCR)-dependent signaling pathways. We previously showed that dihydropyridine receptors (DHPR) involved in TCR-induced calcium inflow were selectively expressed in Th2 cells. In this report, we studied whether cGMP-dependent protein kinase G (PKG) activation was implicated in the regulation of DHPR-dependent calcium response and cytokine production in Th2 lymphocytes. The contribution of cGMP in Th2 signaling was supported by the following results: 1) TCR activation elicited cGMP production, which triggered calcium increase responsible for nuclear factor of activated T cell translocation and Il4 gene expression; 2) guanylate cyclase activation by nitric oxide donors increased intracellular cGMP concentration and induced calcium inflow and IL-4 production; 3) reciprocally, guanylate cyclase inhibition reduced calcium response and Th2 cytokine production associated with TCR activation. In addition, DHPR blockade abolished cGMP-induced [Ca2+]i increase, indicating that TCR-induced DHP-sensitive calcium inflow is dependent on cGMP in Th2 cells. Th2 lymphocytes from PKG1-deficient mice displayed impaired calcium si...Continue Reading

References

Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B BielekovaR Martin
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C M FangerM D Cahalan
Feb 29, 2000·The Journal of Biological Chemistry·L H JiangD Wray
Feb 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J UrbanW C Gause
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M SavignacL Pelletier
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T TamuraH Nariuchi
Sep 29, 2001·Nature Immunology·C Bogdan
Nov 3, 2001·The Journal of Allergy and Clinical Immunology·D M Essayan
Jan 29, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thomas A WhiteTimothy F Walseth
Apr 23, 2003·Immunity·Joaquín Madrenas
Jun 6, 2003·Current Opinion in Immunology·Monte M WinslowGerald R Crabtree
Sep 5, 2003·The Journal of Biological Chemistry·Maya F KotturiWilfred A Jefferies
Nov 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mustapha FaroudiSalvatore Valitutti
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Magali SavignacLucette Pelletier
Jul 30, 2004·Nature Immunology·Martin Spitaler, Doreen A Cantrell
Oct 8, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hilde AbrahamsenKjetil Taskén
Nov 18, 2004·Immunological Reviews·Kerri A Mowen, Laurie H Glimcher
Jun 25, 2005·The Journal of Biological Chemistry·Masakatsu YamashitaToshinori Nakayama
Jan 13, 2006·British Journal of Pharmacology·Victoria Boswell-SmithClive P Page

❮ Previous
Next ❯

Citations

Mar 6, 2007·Pflügers Archiv : European journal of physiology·Magali SavignacJose R Naranjo
Apr 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michael FöllerRobert Feil
May 1, 2008·The Journal of Biological Chemistry·Shane MierschBulent Mutus
Feb 6, 2007·Biochemical Pharmacology·David J Triggle
Jun 13, 2006·Trends in Pharmacological Sciences·Maya F KotturiWilfred A Jefferies
May 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stéphane Leung-Theung-LongSylvie Guerder
Mar 10, 2007·American Journal of Respiratory and Critical Care Medicine·Bruno GomesLucette Pelletier

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.