PMID: 12782039Jun 5, 2003Paper

The changes in crosslink contents in tissues after formalin fixation

Analytical Biochemistry
Masashi AbeAkira Nagano

Abstract

The aim of this study was to detect crosslinks of collagen and elastin in formalin-fixed tissue, to perform quantification of these crosslinks, and to investigate the effects of formalin fixation on crosslink contents in human yellow ligament and cartilage. Pyridinoline (Pyr) is a stable and nonreducible crosslink of collagen. Pentosidine (Pen) is a senescent crosslink formed between arginine and lysine in matrix proteins, including collagen. Desmosine (Des) and its isomer isodesmosine (Isodes) are crosslinks specifically found in elastin. It is useful to measure crosslink contents of collagen and elastin as a way of investigating the properties of various tissues or their pathological changes. If it is possible to evaluate crosslinks of collagen and elastin in formalin-fixed tissues, we can investigate crosslinks in a wide variety of tissues. We used HPLC to compare the concentrations of Pyr, Pen, Des, and Isodes in the formalin-fixed tissues with their concentrations in the frozen tissues. Pyr and Pen were detected in both the formalin-fixed yellow ligament and the cartilage, and their concentrations were not significantly affected by or related to the duration of formalin fixation. Des and Isodes were detected in the formali...Continue Reading

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Citations

Feb 3, 2012·Medical Engineering & Physics·Hanno SteinkeNiels Hammer
Mar 24, 2016·Journal of Anatomy·Frieder KrauseWinfried Banzer
Apr 4, 2014·Acta Odontologica Scandinavica·Takashi MatsuuraHironobu Sato
Nov 3, 2015·Surgical and Radiologic Anatomy : SRA·Jan WilkeWinfried Banzer
Oct 15, 2013·Journal of the Mechanical Behavior of Biomedical Materials·Niels HammerHanno Steinke
Mar 16, 2017·Journal of Biomedical Optics·Lassi RieppoSimo Saarakkala
Jun 14, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Camille BrasseletAntoine Lafont
Mar 30, 2011·Physics in Medicine and Biology·M F G WoodI A Vitkin
Nov 11, 2017·Journal of Applied Physiology·Jan WilkeWinfried Banzer
Oct 23, 2018·ACS Applied Materials & Interfaces·Shelley D RawsonSarah H Cartmell

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