PMID: 11342048May 9, 2001Paper

The chaperone-like activity of a small heat shock protein is lost after sulfoxidation of conserved methionines in a surface-exposed amphipathic alpha-helix

Biochimica Et Biophysica Acta
U HärndahlC Sundby

Abstract

The small heat shock proteins (sHsps) possess a chaperone-like activity which prevents aggregation of other proteins during transient heat or oxidative stress. The sHsps bind, onto their surface, molten globule forms of other proteins, thereby keeping them in a refolding competent state. In Hsp21, a chloroplast-located sHsp in all higher plants, there is a highly conserved region forming an amphipathic alpha-helix with several methionines on the hydrophobic side according to secondary structure prediction. This paper describes how sulfoxidation of the methionines in this amphipathic alpha-helix caused conformational changes and a reduction in the Hsp21 oligomer size, and a complete loss of the chaperone-like activity. Concomitantly, there was a loss of an outer-surface located alpha-helix as determined by limited proteolysis and circular dichroism spectroscopy. The present data indicate that the methionine-rich amphipathic alpha-helix, a motif of unknown physiological significance which evolved during the land plant evolution, is crucial for binding of substrate proteins and has rendered the chaperone-like activity of Hsp21 very dependent on the chloroplast redox state.

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Citations

Dec 21, 2012·Journal of Experimental Botany·Elizabeth R Waters
Jan 1, 2002·The Arabidopsis Book·Ruth Grene
Jun 15, 2007·Protein Science : a Publication of the Protein Society·Emma AhrmanCecilia Sundby Emanuelsson
Feb 1, 2005·Biochimica Et Biophysica Acta·Michael J Davies
Feb 1, 2005·Biochimica Et Biophysica Acta·Cecilia SundbyDenis J Murphy
Oct 24, 2007·Current Topics in Developmental Biology·Derek B Oien, Jackob Moskovitz
Feb 1, 2005·Biochimica Et Biophysica Acta·Alfred HanselToshinori Hoshi
Nov 7, 2007·Rapid Communications in Mass Spectrometry : RCM·Sanjay VenkateshJoshua S Sharp
Feb 1, 2011·Protein Science : a Publication of the Protein Society·Wietske LambertCecilia Emanuelsson
Dec 2, 2014·Biomolecular Concepts·Benjamin Dennis Eisenhardt
Oct 1, 2017·BMC Bioinformatics·Juan C AledoFrancisco J Veredas
Dec 1, 2006·The Journal of Biological Chemistry·Nicolas RouhierSandrine Boschi-Muller
Dec 7, 2019·The Journal of Biological Chemistry·Erin E Nicklow, Carolyn S Sevier

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