PMID: 11327515May 1, 2001Paper

The characterisation and uptake of paraquat in cultured baboon kidney proximal tubule cells (bPTC)

Human & Experimental Toxicology
R MachaalaniG G Duggin

Abstract

A primary culture of baboon proximal tubule cells (bPTC) was prepared and characterised using LLC-PK1 cells of proximal tubule origin and MDCK cells of distal tubule origin, as positive and negative references, respectively. The proximal tubular origin of the bPTC was determined by morphological studies, immunoperoxidase staining and the expression of proximal tubule markers alkaline phosphatase and gammaglutamyltransferase. The hypothesis that paraquat (PQ) is transported by the bPTC was investigated. The cytotoxic threshold for PQ in these cells was determined and compared to the LLC-PK1 and MDCK cells. Furthermore, this study investigated the transport of the monovalent cation tetraethyl ammonium (TEA) and the polyvalent cation cimetidine in the bPTC and demonstrated their effect on the cellular uptake of PQ. The cytotoxic threshold of PQ in the bPTC, determined by cellular viability studies using the method of Trypan blue exclusion, is 0.05 mM at 2 h incubation. The LC50 after 24 h is 76, 61 and 455 microM for the bPTC, LLC-PK1 and MDCK cells, respectively. This indicates that proximal tubule cells are more susceptible to PQ toxicity compared to distal tubule cells, which is consistent with clinical PQ toxicity where renal ...Continue Reading

References

Aug 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L GuesdonS Avrameas
Feb 1, 1979·Archives of Internal Medicine·N D VaziriS M Rosen
Feb 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S G ChangY M Rustum
Sep 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K A Elliget, B F Trump
Dec 30, 1988·Biochemical and Biophysical Research Communications·P B HatzingerJ L Stevens
Sep 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M D AleoP J Kostyniak
Jul 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C L BellC R Scriver
Feb 1, 1985·The Journal of Urology·A L TrifillisB F Trump
May 1, 1985·The American Journal of Physiology·M KinoA Aviv
Oct 1, 1986·Biochemical Medicine and Metabolic Biology·B StatesS Segal
Jul 1, 1988·Journal of Electron Microscopy Technique·J I Kreisberg, P D Wilson
Jan 1, 1988·Renal Physiology and Biochemistry·G J Gstraunthaler
Feb 1, 1984·Kidney International·C J DetrisacD A Sens
Jan 1, 1994·Kidney International·I Van der BiestM E De Broe
Dec 1, 1996·Human & Experimental Toxicology·B S ChanG G Duggin

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Citations

Dec 17, 2008·Biochemical and Biophysical Research Communications·Md Tozammel HoqueSusan P C Cole
Sep 19, 2003·Seminars in Nephrology·Terry R Van Vleet, Rick G Schnellmann
Nov 7, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Su-Ji KimSae-Yong Hong
Oct 14, 2005·Nephrology·Hyo W GilSae Y Hong
Oct 16, 2009·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Hyo-Wook GilSae-Yong Hong
Dec 11, 2007·Free Radical Biology & Medicine·Mohamed SamaiPrabal K Chatterjee
Jul 23, 2004·Physiological Reviews·Stephen H Wright, William H Dantzler
Jul 16, 2019·The Journal of International Medical Research·Yue SongYuhong Tao
Jan 1, 2012·World Journal of Emergency Medicine·Zhi-Jian ZhangCong-Yang Zhou

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