The characteristics of arginine transport by rat cerebellar and cortical synaptosomes

Neurochemical Research
C R Aldridge, K J Collard

Abstract

The uptake of L-[3H]arginine into synaptosomes prepared from rat cerebellum and cortex occurred by a high-affinity carrier-mediated process. The uptake of arginine appeared to be potentiated by removal of extracellular Na+, inhibited by high levels of extracellular K+, but not by depolarization with veratridine or 4-amino pyridine. The effect of Na+ removal or K+ elevation did not seem to be due to changes in intracellular Ca2+ or pH. In both brain regions, uptake was significantly inhibited by L-arginine, L-lysine, L-ornithine, and L-homoarginine, but not by D-arginine nor L-citrulline. Uptake was also inhibited by NG-monomethyl-L-arginine acetate, but not by NG-nitro-L-arginine methyl ester nor NG-nitro-L-arginine except in the cortex at a concentration of 1 mM. The results indicate that the carrier system operating in synaptosomes showed many of the characteristics of the ubiquitous y+ system seen in many other tissues, although its apparent sensitivity to variations in extracellular Na+ was unusual.

References

Jan 1, 1976·The Journal of General Physiology·W F Boron, P De Weer
Jan 1, 1992·Neuroscience·S R Vincent, H Kimura
Apr 30, 1990·Biochemical and Biophysical Research Communications·U FörstermannF Murad
Jul 1, 1990·The Biochemical Journal·R G KnowlesS Moncada
Feb 1, 1991·Trends in Neurosciences·J Garthwaite
Oct 22, 1991·The Journal of Comparative Neurology·H NakamuraS Nakagawa
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R G KnowlesS Moncada
Sep 1, 1987·The American Journal of Physiology·O BussolatiG C Gazzola
Jun 1, 1982·The Journal of General Physiology·D A Nachshen, M P Blaustein
May 1, 1993·The Journal of Comparative Neurology·J G ValtschanoffH H Schmidt
Jun 1, 1994·Journal of Neurophysiology·S Sánchez-ArmassR J Gillies
Jun 1, 1994·Journal of Neurophysiology·R Martínez-ZaguilánS Sánchez-Armass
Jul 1, 1994·Glia·A Schmidlin, H Wiesinger
Jan 15, 1993·Brain Research. Developmental Brain Research·K J CollardY Liu
Jan 7, 1993·Nature·T V Bliss, G L Collingridge

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Citations

Jul 18, 2001·Brain Research. Molecular Brain Research·O BraissantC Bachmann
Jul 17, 1999·Brain Research. Molecular Brain Research·O BraissantC Bachmann
Mar 29, 2001·Progress in Neurobiology·H Wiesinger
Mar 21, 2008·Neurochemical Research·Rocío SalcedaGustavo Sánchez-Chávez

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