The characteristics of Ishikawa endometrial cancer cells are modified by substrate topography with cell-like features and the polymer surface

International Journal of Nanomedicine
Li Hui TanJ J Evans

Abstract

Conventional in vitro culture studies on flat surfaces do not reproduce tissue environments, which have inherent topographical mechanical signals. To understand the impact of these mechanical signals better, we use a cell imprinting technique to replicate cell features onto hard polymer culture surfaces as an alternative platform for investigating biomechanical effects on cells; the high-resolution replication of cells offers the micro- and nanotopography experienced in typical cell-cell interactions. We call this platform a Bioimprint. Cells of an endometrial adenocarcinoma cell line, Ishikawa, were cultured on a bioimprinted substrate, in which Ishikawa cells were replicated on polymethacrylate (pMA) and polystyrene (pST), and compared to cells cultured on flat surfaces. Characteristics of cells, incorporating morphology and cell responses, including expression of adhesion-associated molecules and cell proliferation, were studied. In this project, we fabricated two different topographies for the cells to grow on: a negative imprint that creates cell-shaped hollows and a positive imprint that recreates the raised surface topography of a cell layer. We used two different substrate materials, pMA and pST. We observed that cells ...Continue Reading

Citations

Jul 27, 2021·Frontiers in Cell and Developmental Biology·Annette AffolterNicole Rotter

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Methods Mentioned

BETA
Protein Assay
electrophoresis

Software Mentioned

pST
pMA
ImageJ

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