The characterization of amphibian nucleoplasmins yields new insight into their role in sperm chromatin remodeling.

BMC Genomics
Lindsay J FrehlickJuan Ausio

Abstract

Nucleoplasmin is a nuclear chaperone protein that has been shown to participate in the remodeling of sperm chromatin immediately after fertilization by displacing highly specialized sperm nuclear basic proteins (SNBPs), such as protamine (P type) and protamine-like (PL type) proteins, from the sperm chromatin and by the transfer of histone H2A-H2B. The presence of SNBPs of the histone type (H type) in some organisms (very similar to the histones found in somatic tissues) raises uncertainty about the need for a nucleoplasmin-mediated removal process in such cases and poses a very interesting question regarding the appearance and further differentiation of the sperm chromatin remodeling function of nucleoplasmin and the implicit relationship with SNBP diversity The amphibians represent an unique opportunity to address this issue as they contain genera with SNBPs representative of each of the three main types: Rana (H type); Xenopus (PL type) and Bufo (P type). In this work, the presence of nucleoplasmin in oocyte extracts from these three organisms has been assessed using Western Blotting. We have used mass spectrometry and cloning techniques to characterize the full-length cDNA sequences of Rana catesbeiana and Bufo marinus nucl...Continue Reading

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Citations

Aug 14, 2008·Journal of Proteome Research·Kristie L RoseJuan Ausió
Jun 3, 2009·Reproduction, Fertility, and Development·Olga MudrakAndrei Zalensky
Aug 25, 2009·Reproduction, Fertility, and Development·Sangho RohSsang-Goo Cho
Dec 15, 2010·Biochemistry Research International·Mikael S Lindström
Sep 13, 2016·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ciro Rivera-CasasJose M Eirin-Lopez
Dec 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Lindsay J FrehlickJuan Ausió
Jan 10, 2009·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Kathryn KurtzManel Chiva
Sep 13, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ron M FinnJuan Ausió
Jul 11, 2014·Biology of Reproduction·Aurélien BouleauJulien Bobe
Aug 30, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·José María Eirín-LópezJuan Ausió
Nov 11, 2019·Cell Stress & Chaperones·Ashish Kumar, Dileep Vasudevan
Aug 28, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·David J LópezSonia Bañuelos
Oct 12, 2007·Biochemistry·Lindsay J FrehlickJuan Ausió
Apr 15, 2010·Biochemistry·Amit ThakarJordanka Zlatanova

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Datasets Mentioned

BETA
DQ340657
DQ340656

Methods Mentioned

BETA
protein exchange
antisense oligonucleotides
dot blot
PCR
dot
electrophoresis
Bio-Dot

Software Mentioned

CLUSTAL W
MEGA

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