The characterization of the soybean polygalacturonase-inhibiting proteins (Pgip) gene family reveals that a single member is responsible for the activity detected in soybean tissues

Planta
R D'OvidioF Favaron

Abstract

Polygalacturonase-inhibiting proteins (PGIPs) are leucine-rich repeat (LRR) proteins that inhibit fungal endopolygalacturonases (PGs). They are encoded by multigene families whose members show functional redundancy and subfunctionalization for recognition of fungal PGs. In order to expand the information on the structure and functional features of legume PGIP, we have isolated and characterized four members of the soybean Pgip gene family and determined the properties of the encoded protein products. Sequence analysis showed that these genes form two clusters: one cluster of about 5 kbp containing Gmpgip1 and Gmpgip2, and the other containing Gmpgip3 and Gmpgip4 within a 60 kb fragment of a separate BAC clone. Sequence diversification of the four members resides mainly in the xxLxLxx region that includes residues forming the beta-sheet B1. When compared with other legume Pgip genes, Gmpgip3 groups with the bean genes Pvpgip1 and Pvpgip2, suggesting that these genes are closer to the ancestral gene. At the protein level, only GmPGIP3 shows the capability to inhibit fungal PGs. The spectrum of inhibition of GmPGIP3 against eight different fungal PGs mirrors that of the PGIP purified from soybean tissues and is similar to that of ...Continue Reading

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Citations

Apr 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Manuela CasasoliGiulia De Lorenzo
Jul 15, 2011·Molecular Plant-microbe Interactions : MPMI·Osman RadwanSteven J Clough
Feb 14, 2014·Journal of Natural Products·Lorena Ramos Freitas de SousaPaulo Cezar Vieira
Aug 15, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Michela JanniRenato D'Ovidio
Feb 25, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Anna FarinaRenato D'Ovidio
Jun 6, 2009·Seminars in Cell & Developmental Biology·Stijn LagaertGuido Volckaert
Jun 16, 2006·Trends in Plant Science·Nathalie Juge
Dec 10, 2014·Functional & Integrative Genomics·Aiyun WangZengyan Zhang
Jul 2, 2016·Molecular Plant-microbe Interactions : MPMI·Silvio TundoRenato D'Ovidio
Mar 14, 2012·Acta Biochimica Et Biophysica Sinica·S Ashok PrabhuH S Shetty
Jul 8, 2021·Plant Cell Reports·Reena Deshmukh, Sharad Tiwari

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