PMID: 15228088Jul 2, 2004Paper

The chemical mechanism of action of glucose oxidase from Aspergillus niger

Molecular and Cellular Biochemistry
G WohlfahrtV Leskovac

Abstract

Glucose oxidase from Aspergillus niger (EC 1.1.3.4) is able to catalyze the oxidation of beta-D-glucose with p-benzoquinone, methyl-1,4-benzoquinone, 1,2-naphthoquinone, 1,2-naphthoquinone-4-sulfonic acid, potassium ferricyanide, phenazine methosulfate, and 2,6-dichloroindophenol. In this work, the steady-state kinetic parameters, V1/K(B), for reactions of these substrates were collected from pH 2.5-8. Further, the molecular models of the enzyme's active site were constructed for the free enzyme in the oxidized state, the complex of beta-D-glucose with the oxidized enzyme, the complex of reduced enzyme with methyl-1,4-benzoquinone, the reduced enzyme plus 1,2-naphthoquinone-4-sulfonic acid, oxidized enzyme plus reduced 1,2-naphthoquinone-4-sulfonic acid (hydroquinone anion), and oxidized enzyme plus fully reduced 1,2-naphthoquinone-4-sulfonic acid. Combining the steady-state kinetic and structural data, it was concluded that Glu412 bound to His559, in the active site of enzyme, modulates powerfully its catalytic activity by affecting all the rate constants in the reductive and the oxidative half-reaction of the catalytic cycle. His516 is the catalytic base in the oxidative and the reductive part of the catalytic cycle. It was e...Continue Reading

Citations

Mar 12, 2008·Applied Microbiology and Biotechnology·Chun Ming WongXiao Dong Chen
Feb 2, 2012·Biotechnology Journal·Liliya G VasilchenkoMikhail L Rabinovich
Oct 31, 2012·International Journal of Molecular Sciences·Nurşen SarıNurdan Kurnaz Yetim
Jan 16, 2020·Plants·Sergey KhorobrykhEsa Tyystjärvi
Mar 17, 2017·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Sabrina SchejaEvgeny Katz
Jan 18, 2012·Applied Microbiology and Biotechnology·Aitor Hernández-OrtegaAngel T Martínez
Aug 28, 2015·Scientific Reports·Hiromi YoshidaKoji Sode
Apr 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·David G MíguezIrving R Epstein
Jul 13, 2011·Biotechnology Letters·Kazushige MoriKoji Sode
Oct 21, 2011·Chembiochem : a European Journal of Chemical Biology·Thanyaporn WongnatePimchai Chaiyen
Mar 26, 2009·Biochemistry·Methinee ProngjitPimchai Chaiyen
Mar 26, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Giuseppe ArrabitoBruno Pignataro
Jun 9, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Kenan P Fears, Robert A Latour
Jul 30, 2020·Journal of Environmental Management·Shobana RaviHubert Cabana
Apr 29, 2019·Bioelectrochemistry·Nicolas Mano
Feb 8, 2005·The International Journal of Biochemistry & Cell Biology·V LeskovacD Pericin
Dec 13, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Clive L BaveghemsChalla V Kumar
Apr 6, 2012·Analytical Chemistry·Jan LankelmaGeorge M Whitesides
Apr 9, 2009·Bioelectrochemistry·Soren Demin, Elizabeth A H Hall

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.