PMID: 1181262Jun 1, 1975Paper

[The chemistry of a chitin-protein from crayfish (Astacus fluviatilis) (author's transl)].

Hoppe-Seyler's Zeitschrift für physiologische Chemie
K H HerzogM Liefländer

Abstract

A chitin-protein complex is obtained from crayfish (Astacus fluviatilis) by gentle decalcification with acetic acid and EDTA. The complex is treated with lithium rhodanide, urea, anhydrous formic acid, pronase, papain, anhydrous formamide or 1N NaOH. The first three of these substances have little or no effect on the stability of the chitin-protein complex. The enzymes remove most of the protein, and the last two reagents remove all of it. The protein remaining bound to the polysaccharide after treatment of the chitin-protein complex with pronase or papain is relatively rich in glycine. Quantitative analysis yielded values for the acetyl, glucosaminyl and amino acid residues which reproduce the composition of the corresponding chitin-protein complex. In these calculations however, allowance must be made for the fact that glucosamine is partly destroyed by the acid by hydrolysis and interferes with the determination of basic amino acids. The results of the present work suggest that the chitin in crayfish is present in the form of a stable complex with protein, possibly held together by covalent binding of the protein to the chitin, with glycine as the connecting amino acid.

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