The chemoenzymatic synthesis of the core trisaccharide of N-linked oligosaccharides using a recombinant beta-mannosyltransferase

Carbohydrate Research
G M WattS L Flitsch

Abstract

The chemical synthesis of the beta-mannosyl linkage of N-glycans has presented a great challenge to synthetic carbohydrate chemists. We have therefore investigated the application of beta-mannosyltransferases to the preparative synthesis N-linked core oligosaccharides. In this paper we report the chemoenzymatic synthesis of beta-D-mannopyranosyl-(1-->4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl- (1-->4)-2-acetamido-2-deoxy-alpha-D-glucopyranose on a preparative scale using a phytanyl-linked acceptor in the presence of a recombinant beta-(1-->4)-mannosyltransferase.

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Citations

Jun 29, 2000·Biochemical and Biophysical Research Communications·A C LellouchS L Flitsch
Oct 23, 2016·Biochimica Et Biophysica Acta. General Subjects·Sheng-Tao LiXiao-Dong Gao
Oct 22, 2016·European Journal of Medicinal Chemistry·Michaël BoscoChristine Gravier-Pelletier
Apr 3, 2003·Chembiochem : a European Journal of Chemical Biology·Ines SprungSabine L Flitsch
Jan 19, 2001·Angewandte Chemie·Oyekanmi NashiruStephen G Withers
Jul 3, 2020·Frontiers in Chemistry·Qiang ChaoXiao-Dong Gao
Jan 4, 2003·Chemical Communications : Chem Comm·Ines SprungSabine L Flitsch
Mar 3, 2006·Glycobiology·Eranthie Weerapana, Barbara Imperiali
Jul 14, 2011·Chemical Reviews·Ryan M SchmaltzChi-Huey Wong

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