The chemotaxis response regulator CheY can catalyze its own acetylation

Journal of Molecular Biology
Rina BarakM Eisenbach

Abstract

One of the processes by which CheY, the excitatory response regulator of chemotaxis in Escherichia coli, can be activated to generate clockwise flagellar rotation is by acetyl-CoA synthetase (Acs)-mediated acetylation. Deletion of Acs results in defective chemotaxis, indicating the involvement of Acs-mediated acetylation in chemotaxis. To investigate whether Acs is the sole acetylating agent of CheY, we purified the latter from a delta acs mutant. Mass spectrometry analysis revealed that this protein is partially acetylated in spite of the absence of Acs, suggesting that CheY can be post-translationally acetylated in vivo by additional means. Using [14C]AcCoA in the absence of Acs, we demonstrated that one of these means is autoacetylation, with AcCoA serving as an acetyl donor and with a rate similar to that of Acs-mediated acetylation. Biochemical characterization of autoacetylated CheY and mass spectrometry analysis of its tryptic digests revealed that its acetylated lysine residues are those found in CheY acetylated by Acs, but the acetylation-level distribution among the acetylation sites was different. Like CheY acetylated by Acs, autoacetylated CheY could be deacetylated by Acs. Also similarly to the case of Acs-mediated...Continue Reading

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Citations

Jul 16, 2013·Journal of Bacteriology·Linda I HuAlan J Wolfe
Jan 13, 2010·BMC Systems Biology·Rebecca HamerCharlotte M Deane
Sep 22, 2015·Proteomics·Tassadit OuidirJulie Hardouin
Feb 2, 2010·Current Opinion in Microbiology·Alan J Wolfe
Feb 1, 2008·Journal of Molecular Biology·Jianshe YanMichael Eisenbach
Jul 13, 2012·Journal of Cellular Biochemistry·Longxiang XieJianping Xie
Jun 16, 2011·Proteomics·Joshua D Jones, C David O'Connor
May 22, 2010·Molecular Microbiology·Linda I HuAlan J Wolfe
Apr 20, 2010·Molecular Microbiology·Orna LiarziMichael Eisenbach
Mar 19, 2014·New Biotechnology·Vicente BernalManuel Cánovas
Nov 13, 2014·Molecular Microbiology·Milana FraibergMichael Eisenbach
Jul 9, 2013·Molecular Cell·Brian T WeinertChunaram Choudhary
Aug 3, 2013·FEMS Microbiology Letters·Ru LiJiao-Yu Deng
Sep 8, 2011·Acta Biochimica Et Biophysica Sinica·Ru LiXianen Zhang
May 26, 2017·FEBS Letters·Szilvia Baron, Michael Eisenbach
Apr 26, 2017·Journal of Bacteriology·Valerie J Carabetta, Ileana M Cristea
Jul 17, 2015·Microbiology and Molecular Biology Reviews : MMBR·Kristy L Hentchel, Jorge C Escalante-Semerena
Aug 22, 2007·Journal of Cellular Physiology·Michael Eisenbach
Mar 15, 2019·Emerging Microbes & Infections·Jie RenYu-Feng Yao
Jan 1, 2017·Acta Biochimica Et Biophysica Sinica·Jing GuJiaoyu Deng
Jul 11, 2018·Journal of Oral Microbiology·Yuqing LiMargaret J Duncan
Jul 18, 2015·Microbiology Spectrum·Alan J Wolfe
Aug 25, 2021·MSystems·Jackson Luu, Valerie J Carabetta

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