PMID: 9174973Apr 1, 1997Paper

The Chinese herbal medicine, shinpi-to, inhibits IgE-mediated leukotriene synthesis in rat basophilic leukemia-2H3 cells

Journal of Ethnopharmacology
Y HamasakiS Miyazaki

Abstract

We examined the action of Shinpi-To (Formula divinita; TJ-85), a granular extract of seven Chinese medicinal herbs that is used in treating childhood asthma, on the leukotriene synthesis in rat basophilic leukemia-2H3 cells (RBL-2H3 cells). IgE-loaded cells were stimulated with anti-IgE serum in the presence or absence of Shinpi-To. Released LTC4 and LTB4 were measured by radioimmunoassay (RIA). Shinpi-To significantly inhibited IgE-mediated synthesis of leukotriene (LT)C4 and LTB4. To identify the inhibitory sites, we investigated the action of this extract on four synthetic enzymes, phospholipase A2 (PLA2), 5-lipoxygenase (5-LO). LTC4 synthase, and LTA4 hydrolase. Shinpi-To inhibited the A23187-stimulated release of [3H]arachidonic acid (AA) from the cell membrane, reflecting an effect on PLA2 activity. It also suppressed production of LTC4 and LTB4 when cell lysates were incubated with AA as substrate. It did not inhibit the production of LTC4 and LTB4 when LTA4-free acid was used as the substrate. Shinpi-To did not inhibit the IgE-mediated increase of intracellular Ca2+ ([Ca2+]i) concentration. Results indicate that Shinpi-To inhibits LT synthesis by inhibiting PLA2 and 5-LO activities without affecting the mobilization of ...Continue Reading

References

Apr 1, 1988·The Journal of Pharmacy and Pharmacology·H InoueY Koshihara
Mar 27, 1985·Biochimica Et Biophysica Acta·Y Hamasaki, H H Tai
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S E DahlénB Dahlén
Jan 17, 1984·Biochimica Et Biophysica Acta·Y KoshiharaT Tatsuno
Jul 1, 1995·American Journal of Respiratory and Critical Care Medicine·J F PenroseK F Austen
Jun 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S MatsumotoS Miyazaki
Jan 1, 1994·International Archives of Allergy and Immunology·Y HamasakiS Miyazaki
Jan 29, 1993·Cell·L L LinR J Davis
Jul 1, 1996·International Archives of Allergy and Immunology·Y HamasakiS Miyazaki
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·V Grant

❮ Previous
Next ❯

Citations

Jul 15, 2003·The American Journal of Chinese Medicine·Jiann-Jong ShenMing-Ling Kuo
May 15, 2012·International Immunopharmacology·Yue HaoXianglan Piao
Mar 17, 1999·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·L Bielory, K Lupoli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.