The Chinese Medicine, Jiedu Recipe, Inhibits the Epithelial Mesenchymal Transition of Hepatocellular Carcinoma via the Regulation of Smad2/3 Dependent and Independent Pathways

Evidence-based Complementary and Alternative Medicine : ECAM
Shufang LiangChangquan Ling

Abstract

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. In China, traditional Chinese herb medicine has been widely used in the treatment of HCC. Jiedu Recipe (JR) is a common used prescription which has shown good results against HCC. However, the exact mechanisms of JR are still unknown. Therefore, we investigated the efficacy of JR on HCC in the current study. JR inhibited the cell viability of both SMMC-7721 and Huh7 cells in both time- and dose-dependent manners. Transwell assay revealed that JR decreased the number of migrated cells of SMMC-7721 cells. JR treatment increased the E-cadherin expression level and decreased the levels of p-Smad2/3 and Smad2/3. Further study showed that JR reversed the effect of TGFβ1 on the expression of E-cadherin, vimentin, N-cadherin, and MMP2/9. JR also significantly inhibited TGFβ1-induced migration and invasion of SMMC-7721 and Huh7 cells determined by wound healing assay and transwell assay. TGFβ1 treatment increased the phosphorylation of Smad2/3, p38 MAPK, JNK, ERK1/2, and Akt in SMMC-7721 cells and pretreatment with JR blocked TGFβ1-induced activation of Smad2/3 and Akt and MAPKs. In conclusion, JR inhibits liver cancer cells migration and invasion throu...Continue Reading

References

Aug 18, 2005·Journal of Cell Science·Aristidis Moustakas, Carl-Henrik Heldin
Nov 18, 2005·Cytokine & Growth Factor Reviews·Anita B RobertsKathleen C Flanders
May 29, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shujing LiuZhiheng Xu
Feb 5, 2013·Archives of Medical Research·Hai-hua ChenJiong Yang
Sep 27, 2013·PloS One·Valentina SancisiAlessia Ciarrocchi
Nov 22, 2014·Journal of Integrative Medicine·Yuan-hui ZhangChen Ling
Jul 1, 2017·Journal of Integrative Medicine·Wan-Fu LinChang-Quan Ling

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Methods Mentioned

BETA
Assay
PCR
electrophoresis
surgical resection

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