The chitin catabolic cascade in the marine bacterium Vibrio furnissii. Characterization of an N,N'-diacetyl-chitobiose transport system.

The Journal of Biological Chemistry
N O KeyhaniS Roseman

Abstract

The disaccharide N,N'-diacetyl-chitobiose, (GlcNAc)2, is critical in chitin dissimilation by Vibrio furnissii and, as reported here, is taken up by a specific permease. Since (GlcNAc)2 is rapidly catabolized by V. furnissii, a non-hydrolyzable thioglycoside analogue was used: methyl beta-N,N'-[3H]diacetyl-thiochitobioside (Me-[3H]TCB). Me-TCB and TCB substitute for (GlcNAc)2 as chemoattractants and inducers of beta-N-acetylglucosaminidase activity. The [3H]Me-TCB uptake system was induced only by (GlcNAc)2 and by (GlcNAc)n that can be converted to (GlcNAc)2. The Km for [3H]Me-TCB uptake was </=1 microM and was not affected by Na+ or K+. Uptake appeared to be unidirectional, and in 0.4 M sucrose (+/- K+) the cells accumulated [3H]Me-TCB until it was depleted from the medium, giving an internal concentration of 0.1 M and an internal/external ratio > 1,000. The only effective inhibitors of uptake were: (GlcNAc)n, n = 2-4 > cellobiose > (GlcNAc)5. In 50% artificial sea water (or sucrose/Na+), [3H]Me-TCB accumulation attained a constant steady state level because of efflux, a Na+-dependent process. The physiological implications of these results are considered.

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Citations

May 25, 2011·Marine Biotechnology·Claudiana P SouzaIrma N G Rivera
Dec 2, 1999·Biochimica Et Biophysica Acta·N O Keyhani, S Roseman
Oct 8, 1999·Trends in Cell Biology·S E Moore
Aug 30, 2001·Applied and Environmental Microbiology·S E ThompsonK Peek
Sep 7, 2001·Journal of Bacteriology·K TillyP Rosa
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·N O Keyhani, S Roseman
Dec 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Xibing Li, Saul Roseman
Feb 26, 2016·Applied Microbiology and Biotechnology·Zhen HuangNemat O Keyhani
Feb 19, 2002·The Journal of Biological Chemistry·Jae Kweon ParkSaul Roseman
Jan 18, 2018·Nature Communications·Anuwat AunkhamBert van den Berg
Jan 24, 2019·Bioprocess and Biosystems Engineering·Shun JiangGuimin Zhang
Jan 10, 2020·MSphere·Zhi WangGabrielle Potocki-Veronese
Nov 7, 2015·Microbiology Spectrum·E Fidelma BoydW Brian Whitaker

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