The chitinase-like protein YKL-40 is secreted by airway epithelial cells at base line and in response to compressive mechanical stress.

The Journal of Biological Chemistry
Jin-Ah ParkDaniel J Tschumperlin

Abstract

The chitinase-like protein YKL-40, encoded by the CHI3L1 gene, is a biomarker and functional effector of chronic inflammatory and allergic diseases. In the lung it is associated with asthma severity and reduced lung function. The cellular sources of YKL-40 in human airways and the mechanisms regulating YKL-40 expression are poorly understood. We previously showed that mechanical stress similar to that experienced during bronchoconstriction triggers epithelial cell signaling through epidermal growth factor receptor (EGFR), fibrotic mediator release, and goblet cell hyperplasia consistent with airway remodeling in asthma. We now show that well differentiated normal human bronchial epithelial cells express CHI3L1 and secrete YKL-40 under base-line culture conditions. Mechanical stress (30-cm H(2)O transcellular compressive stress) applied for 3 h induces CHI3L1 expression by ∼4-fold compared with time matched controls, resulting in increased secretion of YKL-40 by 3.6-fold 24 h after onset of the 3-h stimulus. Inhibition of EGFR or MEK1/2 (ERK kinase) significantly but incompletely attenuates mechanical stress-induced up-regulation of CHI3L1 expression in normal human bronchial epithelial cells. Direct activation of EGFR utilizing...Continue Reading

References

Mar 1, 1987·Journal of Applied Physiology·D J DingP T Macklem
Apr 14, 1995·Science·E A Clark, J S Brugge
Feb 14, 1998·Journal of Applied Physiology·B R WiggsR D Kamm
Apr 3, 1999·British Journal of Cancer·C CintinH J Nielsen
Jan 8, 2000·The Journal of Biological Chemistry·M OwhashiN Hayai
Jun 3, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·B ResslerR D Kamm
Jul 18, 2000·Journal of Hepatology·J S JohansenF Bendtsen
Apr 24, 2001·Arthritis and Rheumatism·J S JohansenM Lotz
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Daniel J TschumperlinJeffrey M Drazen
Apr 4, 2003·International Journal of Colorectal Disease·Ioannis E KoutroubakisElias A Kouroumalis
Aug 23, 2003·The Journal of Biological Chemistry·Michael RehliStefan W Krause
Apr 23, 2004·Nature·Daniel J TschumperlinJeffrey M Drazen
Jul 17, 2004·Annual Review of Biomedical Engineering·Christopher S ChenJoe Tien
Nov 16, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Julia S JohansenIb Jarle Christensen
Sep 15, 2005·The Journal of Allergy and Clinical Immunology·Jack A EliasChun Geun Lee
Oct 20, 2005·The Journal of Biological Chemistry·Anneliese D ReckliesSuzanne M Bernier
Jun 24, 2006·American Journal of Respiratory Cell and Molecular Biology·Eric K ChuJeffrey M Drazen
Sep 7, 2006·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·C N Rathcke, H Vestergaard
Sep 19, 2006·American Journal of Respiratory Cell and Molecular Biology·Guohua ZhenDavid J Erle
Jul 25, 2007·Molecular and Cellular Biology·Seung-Wook KimJa Seok Koo
Aug 25, 2007·American Journal of Respiratory Cell and Molecular Biology·Sima AllahverdianDelbert R Dorscheid
Nov 16, 2007·The New England Journal of Medicine·Geoffrey L ChuppJack A Elias
Dec 7, 2007·The American Journal of Pathology·Jin-Ah ParkKenneth B Adler
Apr 12, 2008·The New England Journal of Medicine·Carole OberGeoffrey L Chupp
Aug 30, 2008·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Surakit PungpapongAndrew P Keaveny
Sep 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Séverine LétuvéMarina Pretolani
Dec 25, 2008·American Journal of Respiratory and Critical Care Medicine·Myung Hyun SohnMin Goo Lee
Jan 27, 2009·American Journal of Respiratory Cell and Molecular Biology·Jin-Ah Park, Daniel J Tschumperlin
Feb 14, 2009·The Journal of International Medical Research·K Huang, L D Wu
Mar 20, 2009·International Archives of Allergy and Immunology·Lim ShuhuiW S Fred Wong
Jun 18, 2009·Current Opinion in Allergy and Clinical Immunology·Dominik HartlJack A Elias
Aug 12, 2009·Biochemical and Biophysical Research Communications·Takeo IwataKatsuhiko Yoshimoto
Jan 9, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nikola KojicDaniel J Tschumperlin

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Citations

May 27, 2011·The New England Journal of Medicine·Christopher L GraingePeter H Howarth
Jan 29, 2011·Journal of Biomedicine & Biotechnology·Manasa KannegantiEmiko Mizoguchi
Feb 15, 2014·Asia Pacific Allergy·Wiparat Manuyakorn
Apr 9, 2013·Cellular Signalling·Mansi PrakashCecilia Eriksson
Mar 27, 2012·Immunologic Research·Lakshmi RamakrishnaMaria Alicia Curotto de Lafaille
Oct 17, 2015·American Journal of Respiratory and Critical Care Medicine·Jessie Nicodemus-JohnsonCarole Ober
Dec 15, 2015·Trends in Pharmacological Sciences·Ramaswamy KrishnanAlastair Stewart
Jul 26, 2012·The Journal of Allergy and Clinical Immunology·Jin-Ah ParkJeffrey M Drazen
Jul 23, 2015·Journal of Proteomics·Kees MeijerHan Roelofsen
Jan 22, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M L GavalaN N Jarjour
May 5, 2012·Biochemical and Biophysical Research Communications·Mi Na KimMyung Hyun Sohn
Aug 4, 2015·Nature Materials·Jin-Ah ParkJeffrey J Fredberg
Jan 1, 2015·Nature Reviews. Disease Primers·Stephen T HolgatePeter D Sly
Nov 7, 2015·Molecular and Cellular Pediatrics·Ines MackDominik Hartl
Sep 3, 2013·Experimental Cell Research·Chunyi LiuJuliy M Perelman
Feb 19, 2014·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Abdullah TutenEngin Oral
Feb 18, 2015·Brain : a Journal of Neurology·Ester CantóManuel Comabella
Aug 3, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Bo LanJin-Ah Park
Jul 5, 2018·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Noussa Ragab El BashaHala Hamdy Shaaban
Oct 14, 2017·The European Respiratory Journal·Jose L GomezUNKNOWN SARP Investigators
Jul 3, 2015·Physiology·Jin-Ah ParkJeffrey M Drazen
May 17, 2019·Respiratory Research·Na LiMinchao Li
Jan 6, 2017·The Journal of Biological Chemistry·Abhishek A Kognole, Christina M Payne
Dec 18, 2015·European Clinical Respiratory Journal·Jakob W HansenVibeke Backer
Jan 7, 2016·Biological Chemistry·Julia KzhyshkowskaIrina Mitrofanova
Dec 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hao TangBing Li
Aug 8, 2020·European Respiratory Review : an Official Journal of the European Respiratory Society·Punnam Chander VeeratiChris L Grainge
Jan 24, 2014·International Journal of Molecular Medicine·Momoko YoshidaHiromichi Yamanishi
Jan 24, 2020·Scientific Reports·Ayşe KılıçScott T Weiss
Jun 9, 2020·Frontiers in Medicine·Kenneth P HoughVictor J Thannickal
Feb 1, 2019·Journal of Engineering and Science in Medical Diagnostics and Therapy·Michael J O'Sullivan, Bo Lan
Dec 2, 2020·Open Biology·Michael J O'SullivanJin-Ah Park

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