The chl a fluorescence intensity is remarkably insensitive to changes in the chlorophyll content of the leaf as long as the chl a/b ratio remains unaffected

Biochimica Et Biophysica Acta
Emine DinçGert Schansker

Abstract

The effects of changes in the chlorophyll (chl) content on the kinetics of the OJIP fluorescence transient were studied using two different approaches. An extensive chl loss (up to 5-fold decrease) occurs in leaves suffering from either an Mg(2+) or SO(4)(2-) deficiency. The effects of these treatments on the chl a/b ratio, which is related to antenna size, were very limited. This observation was confirmed by the identical light intensity dependencies of the K, J and I-steps of the fluorescence rise for three of the four treatments and by the absence of changes in the F(685 nm)/F(695 nm)-ratio of fluorescence emission spectra measured at 77K. Under these conditions, the F(0) and F(M)-values were essentially insensitive to the chl content. A second experimental approach consisted of the treatment of wheat leaves with specifically designed antisense oligodeoxynucleotides that interfered with the translation of mRNA of the genes coding for chl a/b binding proteins. This way, leaves with a wide range of chl a/b ratios were created. Under these conditions, an inverse proportional relationship between the F(M) values and the chl a/b ratio was observed. A strong effect of the chl a/b ratio on the fluorescence intensity was also observ...Continue Reading

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Citations

Aug 15, 2014·Photosynthesis Research·Hazem M KalajiMarek Zivcak
Sep 22, 2015·Journal of Photochemistry and Photobiology. B, Biology·Marek ZivcakSuleyman I Allakhverdiev
May 26, 2016·Photosynthesis Research·Christos Chondrogiannis, George Grammatikopoulos
May 30, 2019·Ecotoxicology·Larisa Vladimirovna Afanasyeva, Tuyana Ayushievna Ayushina
Apr 22, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Rodrigo GómezAnabella F Lodeyro

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