PMID: 2111752Jan 1, 1990Paper

The chloride shift may facilitate oxygen loading and unloading to/from the hemoglobin from the brown bear (Ursus arctos L.)

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
O BrixB Giardina

Abstract

1. The oxygen binding properties of hemolyzed bear blood were studied in 0.1 M Tris and 0.1 M Hepes buffer with respect to the possible effects of temperature, pH, pCO2, 2,3-DPG, and chloride ions. 2. There was a significant Bohr shift with a Bohr factor (delta log P50/delta pH) of the magnitude of -0.5. The temperature sensitivity expressed by the apparent heat of oxygenation minus the heat of oxygen in solution was about -8.1 kcal/mol at pH 7.4. 3. Chloride ions decreased the oxygen affinity in the concentration range 50-200 mM, and there was a marked increase in the co-operativity of oxygenation up to a chloride concentration of about 200 mM. 4. There were no effects of pCO2 and 2,3-DPG in the presence of 200 mM Cl-, while in the absence of Cl-, 2,3-DPG had the same effect as 200 mM Cl- at 37 degrees C and pH 7.4. 5. Our results suggests at least two different binding sites for the chloride ion, one high affinity site which may also bind 2,3-DPG in the absence of chloride, and one or more low affinity sites, which only binds chloride. 6. The results further show, that a chloride shift of about 33 mM may account for as much as a 40% increase in O2 unloading, without taking into account the additional effect of the Bohr shift.

References

Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·B GiardinaO Brix
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·O BrixB Giardina
Apr 10, 1989·FEBS Letters·B GiardinaS G Condò
Mar 5, 1973·Biochemical and Biophysical Research Communications·K Imai, I Tyuma
Jan 15, 1980·Journal of Molecular Biology·M F Perutz, K Imai
May 1, 1965·Journal of Molecular Biology·J MONODJ P CHANGEUX

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Citations

Sep 1, 1995·Journal of Pharmaceutical and Biomedical Analysis·S R KnaubC M Riley
Dec 23, 2011·The Journal of Experimental Biology·Jamie R CraitMerav Ben-David
Mar 6, 2012·European Journal of Internal Medicine·Kenrick BerendRijk O B Gans
Apr 15, 2018·Intensive Care Medicine Experimental·Carmen Andrea PfortmuellerJoerg Christian Schefold
Nov 24, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Inge G RevsbechAngela Fago
Mar 23, 2017·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Inge G Revsbech, Angela Fago
Feb 23, 2021·Global Pediatric Health·Renjithkumar Kalikkot ThekkeveeduPradeep Alur

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