The chloroplast protein import channel Toc75: pore properties and interaction with transit peptides

doi:10.1016/s0006-3495(02)75216-8

PMID: 12124272DOI: 10.1016/s0006-3495(02)75216-8Jul 19, 2002Paper

The chloroplast protein import channel Toc75: pore properties and interaction with transit peptides

Biophysical Journal

S HinnahJ Soll

Get PDF

Abstract

The channel properties of Toc75 (the protein import pore of the outer chloroplastic membrane) were further characterized by electrophysiological measurements in planar lipid bilayers. After improvement of the Toc75 reconstitution procedure the voltage dependence of the channel open probability resembled those observed for other beta-barrel pores. Studies concerning the pore size of the reconstituted Toc75 indicate the presence of a narrow restriction zone corresponding to the selectivity filter and a wider pore vestibule with diameters of approximately 14 A and 26 A, respectively. Interactions between Toc75 and different peptides (a genuine chloroplastic transit peptide, a synthetic peptide resembling a transit peptide, and a mitochondrial presequence) show that Toc75 itself is able to differentiate between these peptides and the recognition is based on both conformational and electrostatic interactions.

References

Cannabinoids inhibit N-type calcium channels in neuroblastoma-glioma cells.

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Mackie, B Hille

Crystal structures explain functional properties of two E. coli porins

Aug 27, 1992·Nature·S W CowanJ P Rosenbusch

The chloroplast-targeting domain of plastocyanin transit peptide can form a helical structure but does not have a high affinity for lipid bilayers

Jul 15, 1992·European Journal of Biochemistry·T EndoM Nakai

The sugar-specific outer membrane channel ScrY contains functional characteristics of general diffusion pores and substrate-specific porins

Sep 1, 1991·Molecular Microbiology·K SchüleinR Benzl

Examination of subconductance levels arising from a single ion channel

Dec 7, 1991·Journal of Theoretical Biology·J A Dani, J A Fox

Chloroplast protein topogenesis: import, sorting and assembly

Nov 13, 1991·Biochimica Et Biophysica Acta·A Douwe de Boer, P J Weisbeek

Chloroplast transit peptides. The perfect random coil?

Jan 14, 1991·FEBS Letters·G von Heijne, K Nishikawa

Role of channels in the fusion of vesicles with a planar bilayer.

Dec 1, 1988·Biophysical Journal·D J Woodbury, J E Hall

Fusion of phospholipid vesicles with a planar membrane depends on the membrane permeability of the solute used to create the osmotic pressure

Feb 1, 1989·The Journal of General Physiology·F S CohenM H Akabas

Domain structure of mitochondrial and chloroplast targeting peptides.

Apr 1, 1989·European Journal of Biochemistry·G von HeijneR G Herrmann

Artificial mitochondrial presequences.

Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D S Allison, G Schatz

Video fluorescence microscopy studies of phospholipid vesicle fusion with a planar phospholipid membrane. Nature of membrane-membrane interactions and detection of release of contents

Nov 1, 1987·The Journal of General Physiology·W D Niles, F S Cohen

Ionic blockage of sodium channels in nerve

Jun 1, 1973·The Journal of General Physiology·A M Woodhull

A simple procedure for removal of Triton X-100 from protein samples

May 1, 1973·Analytical Biochemistry·P W Holloway

Fusion of phospholipid vesicles with planar phospholipid bilayer membranes. I. Discharge of vesicular contents across the planar membrane

Mar 1, 1980·The Journal of General Physiology·J ZimmerbergA Finkelstein

Charges, currents, and potentials in ionic channels of one conformation

May 1, 1993·Biophysical Journal·D Chen, R Eisenberg

A constituent of the chloroplast import complex represents a new type of GTP-binding protein

Mar 1, 1995·The Plant Journal : for Cell and Molecular Biology·M SeedorfJ Soll

Functional domains of the ferredoxin transit sequence involved in chloroplast import.

Feb 24, 1995·The Journal of Biological Chemistry·M PilonB de Kruijff

Identification of two GTP-binding proteins in the chloroplast protein import machinery

Nov 11, 1994·Science·F KesslerD J Schnell

Envelope membrane proteins that interact with chloroplastic precursor proteins

Jan 1, 1994·The Plant Cell·S E Perry, K Keegstra

The secondary structure of the ferredoxin transit sequence is modulated by its interaction with negatively charged lipids

Nov 15, 1993·FEBS Letters·L HorniakB de Kruijff

Colicin import and pore formation: a system for studying protein transport across membranes?

Jun 1, 1995·Molecular Microbiology·C J Lazdunski

Two components of the chloroplast protein import apparatus, IAP86 and IAP75, interact with the transit sequence during the recognition and translocation of precursor proteins at the outer envelope

Jul 1, 1996·The Journal of Cell Biology·Y MaD J Schnell

Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation

Nov 15, 1996·Cell·D HaneinC W Akey

A novel method for structure-based prediction of ion channel conductance properties

Mar 1, 1997·Biophysical Journal·O S SmartM S Sansom

The aqueous pore through the translocon has a diameter of 40-60 A during cotranslational protein translocation at the ER membrane

May 16, 1997·Cell·B D HammanA E Johnson

A folded protein can be transported across the chloroplast envelope and thylakoid membranes

May 1, 1997·Molecular Biology of the Cell·S A Clark, S M Theg

The charge state of an ion channel controls neutral polymer entry into its pore

Jan 1, 1997·European Biophysics Journal : EBJ·S M Bezrukov, J J Kasianowicz

Reconstitution of a chloroplast protein import channel

Feb 21, 1998·The EMBO Journal·S C HinnahJ Soll

Analysis of the interactions of preproteins with the import machinery over the course of protein import into chloroplasts

Feb 7, 1998·The Journal of Cell Biology·A Kouranov, D J Schnell

Interpretation of substates in ion channels: unipores or multipores?

Jan 1, 1997·Progress in Biophysics and Molecular Biology·D R Laver, W G Peter

The preprotein translocation channel of the outer membrane of mitochondria

Jul 11, 1998·Cell·K P KünkeleW Neupert

Refolding of Escherichia coli produced membrane protein inclusion bodies immobilised by nickel chelating chromatography

Aug 26, 1998·FEBS Letters·H RoglI Collinson

Voltage gating is a fundamental feature of porin and toxin beta-barrel membrane channels

Aug 26, 1998·FEBS Letters·G BainbridgeJ H Lakey

Domains of a transit sequence required for in vivo import in Arabidopsis chloroplasts

Oct 10, 1998·Plant Physiology·W A RensinkP Weisbeek

Origin of a chloroplast protein importer

Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·B BölterR Wagner

Physical origin of selectivity in ionic channels of biological membranes

Jan 6, 1999·Biophysical Journal·A Laio, V Torre

The evolutionary origin of the protein-translocating channel of chloroplastic envelope membranes: identification of a cyanobacterial homolog

Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·S ReumannK Keegstra

Mechanisms of solute transport through outer membrane porins: burning down the house

Mar 6, 1999·Current Opinion in Microbiology·P E Klebba, S M Newton

The structural flexibility of the preferredoxin transit peptide

Jul 15, 1999·FEBS Letters·H L WienkB de Kruijff

A coil-helix instead of a helix-coil motif can be induced in a chloroplast transit peptide from Chlamydomonas reinhardtii

Sep 22, 1999·European Journal of Biochemistry·I KrimmJ M Lancelin

Initial binding of preproteins involving the Toc159 receptor can be bypassed during protein import into chloroplasts

Mar 11, 2000·Plant Physiology·K ChenD J Schnell

Toc64, a new component of the protein translocon of chloroplasts

Mar 22, 2000·The Journal of Cell Biology·K Sohrt, J Soll

Solute pores, ion channels, and metabolite transporters in the outer and inner envelope membranes of higher plant plastids

Apr 5, 2000·Biochimica Et Biophysica Acta·H E Neuhaus, R Wagner

Topology studies of the chloroplast protein import channel Toc75

Oct 13, 2000·Biological Chemistry·N SveshnikovaE Schleiff

Travelling of proteins through membranes: translocation into chloroplasts

Oct 13, 2000·Planta·E Schleiff, J Soll

Protein import: the hitchhikers guide into chloroplasts

Nov 15, 2000·Biological Chemistry·U C Vothknecht, J Soll

Assembly of Newly Imported Oxygen-Evolving Complex Subunits in Isolated Chloroplasts: Sites of Assembly and Mechanism of Binding

Mar 1, 1997·The Plant Cell·A. HashimotoS. M. Theg

A consensus nomenclature for the protein-import components of the chloroplast envelope

Aug 1, 1997·Trends in Cell Biology·D J SchnellJ Soll

❮ Previous

Next ❯

Citations

Excursion of a single polypeptide into a protein pore: simple physics, but complicated biology.

Mar 28, 2008·European Biophysics Journal : EBJ·Mohammad M Mohammad, Liviu Movileanu

Characterization of a T-DNA insertion mutant for the protein import receptor atToc33 from chloroplasts

Nov 2, 2004·Molecular Genetics and Genomics : MGG·M GutensohnR B Klösgen

Unimolecular study of the interaction between the outer membrane protein OmpF from E. coli and an analogue of the HP(2-20) antimicrobial peptide.

Feb 25, 2010·Journal of Bioenergetics and Biomembranes·Aurelia ApetreiTudor Luchian

Import of preproteins into the chloroplast inner envelope membrane.

Aug 16, 2008·Plant Molecular Biology·Ewa Firlej-KwokaBettina Bölter

Toc GTPases

Mar 31, 2007·Journal of Biomedical Science·Hsou-Min LiChwan-Deng Hsiao

The Sec61p complex is a dynamic precursor activated channel

Jul 31, 2003·Molecular Cell·Andreas WirthRichard Wagner

Factors governing helix formation in peptides confined to carbon nanotubes

Sep 27, 2008·Nano Letters·Edward P O'BrienBernard R Brooks

Translocation of double-stranded DNA through membrane-adapted phi29 motor protein nanopores.

Nov 7, 2009·Nature Nanotechnology·David WendellPeixuan Guo

Common ground for protein translocation: access control for mitochondria and chloroplasts

Dec 9, 2010·Nature Reviews. Molecular Cell Biology·Enrico Schleiff, Thomas Becker

The import of ferredoxin-NADP+ reductase precursor into chloroplasts is modulated by the region between the transit peptide and the mature core of the protein

Nov 9, 2002·European Journal of Biochemistry·Daniela V RialJorgelina Ottado

Single molecule detection of intermediates during botulinum neurotoxin translocation across membranes

Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Audrey Fischer, Mauricio Montal

Evidence for glycoprotein transport into complex plastids

Jun 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Madeleine PeschkeFranziska Hempel

Toc receptor dimerization participates in the initiation of membrane translocation during protein import into chloroplasts.

Sep 12, 2009·The Journal of Biological Chemistry·Jeonghwa LeeDanny J Schnell

Filling the gap, evolutionarily conserved Omp85 in plastids of chromalveolates.

Jan 1, 2010·The Journal of Biological Chemistry·Lars BullmannEnrico Schleiff

The mitochondrial oxidase assembly protein1 (Oxa1) insertase forms a membrane pore in lipid bilayers.

Jul 26, 2012·The Journal of Biological Chemistry·Vivien KrügerMichael Meinecke

Bacterial origin of a mitochondrial outer membrane protein translocase: new perspectives from comparative single channel electrophysiology.

Jul 11, 2012·The Journal of Biological Chemistry·Anke HarsmanRichard Wagner

The TpsB translocator HMW1B of haemophilus influenzae forms a large conductance channel.

Apr 12, 2008·The Journal of Biological Chemistry·Guillaume DuretAnne H Delcour

A proteomic analysis of the chromoplasts isolated from sweet orange fruits [Citrus sinensis (L.) Osbeck].

Aug 16, 2011·Journal of Experimental Botany·Yunliu ZengXiuxin Deng

A molecular-genetic study of the Arabidopsis Toc75 gene family

May 24, 2005·Plant Physiology·Amy BaldwinPaul Jarvis

Functional characterization of sequence motifs in the transit peptide of Arabidopsis small subunit of rubisco

Dec 31, 2005·Plant Physiology·Dong Wook LeeInhwan Hwang

Characterization of the preprotein and amino acid transporter gene family in Arabidopsis

Nov 14, 2006·Plant Physiology·Monika W MurchaJames Whelan

In vivo analyses of the roles of essential Omp85-related proteins in the chloroplast outer envelope membrane.

Jul 16, 2011·Plant Physiology·Weihua HuangPaul Jarvis

The Arabidopsis ppi1 mutant is specifically defective in the expression, chloroplast import, and accumulation of photosynthetic proteins

Aug 5, 2003·The Plant Cell·Sybille KubisPaul Jarvis

Functional specialization amongst the Arabidopsis Toc159 family of chloroplast protein import receptors

Jul 27, 2004·The Plant Cell·Sybille KubisPaul Jarvis

Arabidopsis nuclear-encoded plastid transit peptides contain multiple sequence subgroups with distinctive chloroplast-targeting sequence motifs.

Jun 17, 2008·The Plant Cell·Dong Wook LeeInhwan Hwang

Tic20 forms a channel independent of Tic110 in chloroplasts.

Oct 4, 2011·BMC Plant Biology·Erika Kovács-BogdánBettina Bölter

Bilateral communication between plastid and the nucleus: plastid protein import and plastid-to-nucleus retrograde signaling

Mar 9, 2010·Bioscience, Biotechnology, and Biochemistry·Takehito Inaba

Structure-function relation of phospholamban: modulation of channel activity as a potential regulator of SERCA activity

Jan 12, 2013·PloS One·Serena SmeazzettoGerhard Thiel

Ligand recognition by the TPR domain of the import factor Toc64 from Arabidopsis thaliana

Jan 7, 2014·PloS One·Rashmi PanigrahiAlice Vrielink

Molecular interactions within the plant TOC complex

Apr 2, 2009·Biological Chemistry·Maik S Sommer, Enrico Schleiff

Anthrax toxin-induced rupture of artificial lipid bilayer membranes

Aug 21, 2013·The Journal of Chemical Physics·Brian J NabloJohn J Kasianowicz

A GTP-driven motor moves proteins across the outer envelope of chloroplasts

Apr 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Enrico SchleiffJürgen Soll

Protein transport into chloroplasts

Mar 3, 2010·Annual Review of Plant Biology·Hsou-min Li, Chi-Chou Chiu

In vivo assessment of the significance of phosphorylation of the Arabidopsis chloroplast protein import receptor, atToc33

Jan 18, 2006·FEBS Letters·Henrik AronssonPaul Jarvis

Let's talk about...chloroplast import

Jan 25, 2005·Plant Biology·T BeckerE Schleiff

Exploring protein import pores of cellular organelles at the single molecule level using the planar lipid bilayer technique

Jun 21, 2011·European Journal of Cell Biology·Anke HarsmanRichard Wagner

Applications of biological pores in nanomedicine, sensing, and nanoelectronics.

Jun 22, 2010·Current Opinion in Biotechnology·Sheereen MajdMichael Mayer

Peroxisomal protein import pores

Oct 27, 2015·Biochimica Et Biophysica Acta·Michael MeineckeRichard Wagner

Development and optimization of an in vitro chloroplastic protein import assay using recombinant proteins

Apr 18, 2008·Plant Physiology and Biochemistry : PPB·Hitoshi InoueMitsuru Akita

Are bacterial 'autotransporters' really transporters?

Oct 16, 2007·Trends in Microbiology·Harris D Bernstein

The chloroplastic protein translocation channel Toc75 and its paralog OEP80 represent two distinct protein families and are targeted to the chloroplastic outer envelope by different mechanisms

Jul 17, 2004·The Plant Journal : for Cell and Molecular Biology·Kentaro Inoue, Daniel Potter

Conserved pore-forming regions in polypeptide-transporting proteins

Mar 9, 2005·The FEBS Journal·Suncana MoslavacEnrico Schleiff

A polyglycine stretch is necessary for proper targeting of the protein translocation channel precursor to the outer envelope membrane of chloroplasts

Jun 6, 2003·The Plant Journal : for Cell and Molecular Biology·Kentaro Inoue, Kenneth Keegstra

The outer membrane of a heterocyst-forming cyanobacterium is a permeability barrier for uptake of metabolites that are exchanged between cells

Aug 26, 2009·Molecular Microbiology·Kerstin NicolaisenEnrique Flores

Targeting of nucleus-encoded proteins to chloroplasts in plants.

Dec 17, 2008·The New Phytologist·Paul Jarvis

Protein unfolding is an essential requirement for transport across the parasitophorous vacuolar membrane of Plasmodium falciparum

Dec 2, 2008·Molecular Microbiology·Nina GehdeJude M Przyborski

Protein transport in organelles: The Toc complex way of preprotein import

Feb 4, 2009·The FEBS Journal·Birgit Agne, Felix Kessler

Molecular architecture and function of the Omp85 family of proteins

Nov 30, 2005·Molecular Microbiology·Ian E GentleTrevor Lithgow

Deletion of core components of the plastid protein import machinery causes differential arrest of embryo development in Arabidopsis thaliana

Jan 26, 2006·Plant Biology·B Hust, M Gutensohn

Multi-functional roles for the polypeptide transport associated domains of Toc75 in chloroplast protein import

Mar 22, 2016·ELife·Yamuna D PailaDanny J Schnell

❮ Previous

Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.