PMID: 7541537Jul 3, 1995Paper

The choice of alternative 5' splice sites in influenza virus M1 mRNA is regulated by the viral polymerase complex

Proceedings of the National Academy of Sciences of the United States of America
S R ShihR M Krug

Abstract

The influenza virus M1 mRNA has two alternative 5' splice sites: a distal 5' splice site producing mRNA3 that has the coding potential for 9 amino acids and a proximal 5' splice site producing M2 mRNA encoding the essential M2 ion-channel protein. Only mRNA3 was made in uninfected cells transfected with DNA expressing M1 mRNA. Similarly, using nuclear extracts from uninfected cells, in vitro splicing of M1 mRNA yielded only mRNA3. Only when the mRNA3 5' splice site was inactivated by mutation was M2 mRNA made in uninfected cells and in uninfected cell extracts. In influenza virus-infected cells, M2 mRNA was made, but only after a delay, suggesting that newly synthesized viral gene product(s) were needed to activate the M2 5' splice site. We present strong evidence that these gene products are the complex of the three polymerase proteins, the same complex that functions in the transcription and replication of the viral genome. Gel shift experiments showed that the viral polymerase complex bound to the 5' end of the viral M1 mRNA in a sequence-specific and cap-dependent manner. During in vitro splicing catalyzed by uninfected cell extracts, the binding of the viral polymerase complex blocked the mRNA3 5' splice site, resulting in...Continue Reading

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Citations

May 23, 2007·Nucleic Acids Research·Eva TorreiraOscar Llorca
Dec 17, 2009·Journal of Virology·Yasushi MurakiSeiji Hongo
Aug 3, 2001·Journal of Virology·S Schultz-CherryV S Hinshaw
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May 16, 2014·MBio·Julia DuboisManuel Rosa-Calatrava
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Mar 16, 2011·The Journal of General Virology·Katja BierErvin Fodor
Sep 16, 2011·The Journal of General Virology·Nicole C Robb, Ervin Fodor
Oct 16, 2012·RNA Biology·Walter N MossDouglas H Turner
Oct 21, 2019·Journal of Biomedical Science·Daria MezhenskayaLarisa Rudenko
Feb 27, 2003·The Journal of General Virology·Svetlana V Bourmakina, Adolfo García-Sastre
Dec 8, 2017·International Journal of Molecular Sciences·Rashid ManzoorAyato Takada
Jan 1, 2019·Cold Spring Harbor Symposia on Quantitative Biology·Matthew G Thompson, Kristen W Lynch
Jul 16, 2014·Biochemistry·Tian JiangDouglas H Turner

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