The cholinergic gene locus in amphioxus: molecular characterization and developmental expression patterns

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Simona CandianiMario Pestarino

Abstract

The cholinergic gene locus (CGL), consisting of the vesicular acetylcholine transporter (VAChT)/choline acetyltransferase (ChAT) gene, encodes two specific cholinergic neuronal markers used extensively to study cholinergic transmission. In the present work, we isolated the amphioxus homologs of VAChT and ChAT and examined their expression during development. Analysis of the 5' untranslated region of VAChT and ChAT suggests that the splicing of the VAChT/ChAT mRNA has been evolutionarily conserved in amphioxus and mammals. By double whole-mount in situ hybridization, we demonstrate that VAChT and ChAT are coexpressed in the same cells. They are first expressed in four pairs of differentiating cells in the neural plate. Their later expression is primarily in the anterior nerve cord in several types of motoneurons, some of the interneurons and in the receptor cells of the larval ocellus.

References

Jan 1, 1991·Progress in Neurobiology·N J Woolf
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S BerrardJ Mallet
Oct 15, 1974·The Journal of Comparative Neurology·P R Flood
Aug 26, 1994·Journal of Molecular Biology·A AlfonsoJ B Rand
May 1, 1994·Journal of Neurochemistry·D Wu, L B Hersh
Apr 1, 1993·Brain Research. Molecular Brain Research·M KengakuT Deguchi
Feb 28, 1998·The Journal of Biological Chemistry·T KitamotoP M Salvaterra
May 29, 1998·Journal of Neurochemistry·L E Eiden
Oct 6, 1999·Current Opinion in Neurobiology·L Z Holland, N D Holland
Mar 18, 2000·Developmental Biology·W R JackmanC B Kimmel
Apr 12, 2002·Journal of Morphology·Thurston C Lacalli
Oct 3, 2002·Evolution & Development·William R Jackman, Charles B Kimmel
Nov 19, 2002·Developmental Biology·Françoise Mazet, Sebastian M Shimeld
Jun 2, 2004·Genesis : the Journal of Genetics and Development·Reiko YoshidaTakehiro Kusakabe
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
May 12, 2007·The Journal of Comparative Neurology·Anne Mette SøviknesJoel C Glover
Nov 29, 2007·The Journal of Comparative Neurology·Janet S DuerrJames B Rand
Mar 12, 2008·Brain Research Bulletin·Thurston C Lacalli

❮ Previous
Next ❯

Citations

Mar 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Manuel IrimiaJordi Garcia-Fernàndez
Feb 2, 2010·BMC Evolutionary Biology·Simona CandianiMario Pestarino
Jun 9, 2012·BMC Neuroscience·Simona CandianiMario Pestarino
Jul 5, 2011·EvoDevo·Simona CandianiMario Pestarino
Feb 7, 2009·Evolution & Development·Stéphanie BertrandHéctor Escrivà
Feb 14, 2015·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Yana MikhalevaDaniel Chourrout
Mar 15, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kota BanzaiSusumu Izumi
Oct 1, 2020·Scientific Reports·Lourdes Mateos-HernandézLadislav Šimo
Jun 3, 2021·International Journal of Molecular Sciences·Veronica CorsettiAda Maria Tata

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.