The chromatin remodeling subunit Baf200 promotes normal hematopoiesis and inhibits leukemogenesis

Journal of Hematology & Oncology
Lulu LiuYan Zhang

Abstract

Adenosine triphosphate (ATP)-dependent chromatin remodeling SWI/SNF-like BAF and PBAF complexes have been implicated in the regulation of stem cell function and cancers. Several subunits of BAF or PBAF, including BRG1, BAF53a, BAF45a, BAF180, and BAF250a, are known to be involved in hematopoiesis. Baf200, a subunit of PBAF complex, plays a pivotal role in heart morphogenesis and coronary artery angiogenesis. However, little is known on the importance of Baf200 in normal and malignant hematopoiesis. Utilizing Tie2-Cre-, Vav-iCre-, and Mx1-Cre-mediated Baf200 gene deletion combined with fetal liver/bone marrow transplantation, we investigated the function of Baf200 in fetal and adult hematopoiesis. In addition, a mouse model of MLL-AF9-driven leukemogenesis was used to study the role of Baf200 in malignant hematopoiesis. We also explored the potential mechanism by using RNA-seq, RT-qPCR, cell cycle, and apoptosis assays. Tie2-Cre-mediated loss of Baf200 causes perinatal death due to defective erythropoiesis and impaired hematopoietic stem cell expansion in the fetal liver. Vav-iCre-mediated loss of Baf200 causes only mild anemia and enhanced extramedullary hematopoiesis. Fetal liver hematopoietic stem cells from Tie2-Cre+, Baf200...Continue Reading

References

Oct 1, 1995·Differentiation; Research in Biological Diversity·J J Lyon, R J Watson
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·R YamanakaK G Xanthopoulos
May 16, 2001·Genesis : the Journal of Genetics and Development·R ConstienB Arnold
Dec 17, 2003·The Journal of Experimental Medicine·Thomas C GebuhrTerry Magnuson
Jul 25, 2006·Nature·Andrei V KrivtsovScott A Armstrong
Feb 26, 2008·Cell·Stuart H Orkin, Leonard I Zon
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jul 21, 2010·Genesis : the Journal of Genetics and Development·Yuefeng TangLucy Liaw
May 25, 2011·Critical Reviews in Oncology/hematology·Carmen VicenteMaría D Odero
May 25, 2011·Haematologica·Fatemehsadat EsteghamatSjaak Philipsen
Nov 19, 2011·Oncotarget·Hong ZhaoJianqiang Cai
Oct 11, 2012·International Journal of Cancer. Journal International Du Cancer·Gilles ManceauHélène Blons
Feb 1, 2013·Blood·Sebastian ThiemeSebastian Brenner
Jun 15, 2013·Oxidative Medicine and Cellular Longevity·Michelle R CampbellDouglas A Bell
Nov 13, 2013·The Journal of Experimental Medicine·Omar Abdel-WahabRoss L Levine
Nov 21, 2013·Blood·Jiapeng WangFeng-Chun Yang
Nov 29, 2013·Genes & Development·Junwei ShiChristopher R Vakoc
Jun 21, 2014·Experimental Hematology·Aldo Ciau-UitzRoger Patient
Aug 19, 2014·Nature·Panagiotis NtziachristosIannis Aifantis
Nov 12, 2014·Annual Review of Pathology·Julien Masliah-PlanchonOlivier Delattre
Mar 15, 2015·Biochemical and Biophysical Research Communications·Tianyuan HuWeiping Yuan
Feb 18, 2016·The Journal of Experimental Medicine·Per HolmfeldtShannon McKinney-Freeman
Sep 30, 2016·Journal of Hematology & Oncology·Xinyu TianShengjun Wang
Jan 7, 2017·Journal of Hematology & Oncology·John E J Rasko, Justin J-L Wong
Feb 10, 2017·Blood·Raman SoodPaul Liu

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Citations

Oct 28, 2019·International Journal of Molecular Sciences·Nashwa El Hadidy, Vladimir N Uversky
Aug 2, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiayi TuMugen Liu
Sep 27, 2020·Cells·Robin LoeschSabine Colnot
Dec 22, 2020·Experimental Hematology·Theresa BluemnNan Zhu
Sep 28, 2021·Frontiers in Immunology·Biao ChenJi-Long Chen

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Datasets Mentioned

BETA
SRP117796

Methods Mentioned

BETA
PBAF
PCR
X-ray
flow cytometry
RNA-seq
genotyping

Software Mentioned

Prism
PrimerBank
FlowJo
Primer3
GraphPad
DAVID Bioinformatics Resources

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