The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription

EMBO Reports
Piergiorgio PercipalleAnn-Kristin Ostlund Farrants

Abstract

Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein-protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.

References

Jan 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·U Scheer, R Benavente
Oct 13, 2000·Science·L Pestic-DragovichP de Lanerolle
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
May 1, 2002·The EMBO Journal·Ludmila BozhenokPatrick Varga-Weisz
Nov 26, 2002·Science·Miroslav DundrTom Misteli
Nov 25, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alexandra Lusser, James T Kadonaga
Feb 26, 2004·Experimental Cell Research·Nathalie Fomproix, Piergiorgio Percipalle
Mar 17, 2004·Biochimica Et Biophysica Acta·Davide F V Corona, John W Tamkun
Jun 16, 2004·Current Opinion in Genetics & Development·Rimma Belotserkovskaya, Danny Reinberg
Jun 16, 2004·Current Opinion in Genetics & Development·Andrew Flaus, Tom Owen-Hughes
Oct 27, 2004·Nature Cell Biology·Wilma A HofmannPrimal de Lanerolle
Nov 24, 2004·Nature Cell Biology·Vlada V PhilimonenkoIngrid Grummt
Dec 3, 2004·Genes & Development·Ping HuNouria Hernandez
Jan 7, 2005·Nature·Jens S AndersenMatthias Mann
Feb 16, 2005·Nature Structural & Molecular Biology·Alexander KukalevPiergiorgio Percipalle

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Citations

Jan 1, 2013·Cellular and Molecular Life Sciences : CMLS·Kei Miyamoto, J B Gurdon
Oct 18, 2008·Chromosoma·Peter C McKeown, Peter J Shaw
Sep 8, 2006·Histochemistry and Cell Biology·M KahleP Hozák
Nov 29, 2008·Histochemistry and Cell Biology·Hana DingováPavel Hozák
May 6, 2010·Histochemistry and Cell Biology·E CastanoP Hozák
Aug 5, 2010·Histochemistry and Cell Biology·V V PhilimonenkoP Hozák
Feb 1, 2011·Histochemistry and Cell Biology·Stefan Hübner, Athina Efthymiadis
Nov 13, 2009·Nucleic Acids Research·Chuong D PhamGavin R Schnitzler
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Neus Visa, Piergiorgio Percipalle
Feb 18, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Chris Barnett, Jocelyn E Krebs
Jan 10, 2012·International Journal of Cell Biology·Masahiro KumetaKunio Takeyasu
Feb 20, 2009·Journal of Cell Science·Wilma A HofmannPrimal de Lanerolle
Nov 20, 2013·Trends in Cell Biology·Prabodh Kapoor, Xuetong Shen
Oct 16, 2012·Biochimica Et Biophysica Acta·Ingrid Grummt, Gernot Längst
Mar 13, 2014·BMC Cell Biology·Neil L SielskiWilma A Hofmann
Mar 21, 2009·Cellular and Molecular Life Sciences : CMLS·Piergiorgio Percipalle
Feb 6, 2016·Histochemistry and Cell Biology·Ildikó KristóPéter Vilmos
Jun 28, 2011·Biochimica Et Biophysica Acta·Fabian ErdelKarsten Rippe
Aug 4, 2011·The FEBS Journal·Fabian Erdel, Karsten Rippe
Jul 18, 2009·Cell Motility and the Cytoskeleton·Andrew J Lindsay, Mary W McCaffrey
Apr 1, 2006·Current Opinion in Cell Biology·Piergiorgio Percipalle, Ann-Kristin Ostlund Farrants
Apr 18, 2015·Nucleic Acids Research·Fatemeh SadeghifarAnn-Kristin Östlund Farrants
Feb 27, 2013·Experimental Cell Research·Ryan S SchwabWilma A Hofmann
May 19, 2016·Cytoskeleton·Ivan V Maly, Wilma A Hofmann
Aug 9, 2016·PLoS Genetics·Randall A DassC Theresa Vincent
Aug 3, 2016·Scientific Reports·Tomáš VenitPavel Hozák

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