The CIF1 protein is a master orchestrator of trypanosome cytokinesis that recruits several cytokinesis regulators to the cytokinesis initiation site

The Journal of Biological Chemistry
Qing ZhouZiyin Li

Abstract

To proliferate, the parasitic protozoan Trypanosoma brucei undergoes binary fission in a unidirectional manner along the cell's longitudinal axis from the cell anterior toward the cell posterior. This unusual mode of cell division is controlled by a regulatory pathway composed of two evolutionarily conserved protein kinases, Polo-like kinase and Aurora B kinase, and three trypanosome-specific proteins, CIF1, CIF2, and CIF3, which act in concert at the cytokinesis initiation site located at the distal tip of the newly assembled flagellum attachment zone (FAZ). However, additional regulators that function in this cytokinesis signaling cascade remain to be identified and characterized. Using proximity biotinylation, co-immunofluorescence microscopy, and co-immunoprecipitation, we identified 52 CIF1-associated proteins and validated six CIF1-interacting proteins, including the putative protein phosphatase KPP1, the katanin p80 subunit KAT80, the cleavage furrow-localized proteins KLIF and FRW1, and the FAZ tip-localized proteins FAZ20 and FPRC. Further analyses of the functional interplay between CIF1 and its associated proteins revealed a requirement of CIF1 for localization of a set of CIF1-associated proteins, an interdependence...Continue Reading

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Citations

Dec 12, 2019·Frontiers in Cellular and Infection Microbiology·Tansy C Hammarton
Jul 29, 2020·The Journal of Biological Chemistry·Felice D KellyScott M Landfear
Oct 23, 2020·PLoS Pathogens·Clare HallidayJack D Sunter
Nov 13, 2020·Molecular Biology of the Cell·Paul C Campbell, Christopher L de Graffenried
Sep 1, 2019·Trends in Parasitology·Amy N Sinclair, Christopher L de Graffenried
Oct 15, 2020·Open Biology·Yasuhiro KurasawaZiyin Li

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