The cigarette smoke component acrolein inhibits expression of the innate immune components IL-8 and human beta-defensin 2 by sinonasal epithelial cells

American Journal of Rhinology
Won Kyung LeeAndrew P Lane

Abstract

Tobacco use is associated with poorer outcomes of medical and surgical therapy for chronic rhinosinusitis (CRS), although the underlying mechanism is unknown. Acrolein (AC) is a major component of cigarette smoke that has been shown to suppress innate immune gene expression by human bronchial epithelial cells and murine macrophages. In this study, we explore whether exposure of human sinonasal epithelial cells (HSNECs) to AC similarly reduces their innate immune gene expression. Primary HSNECs from CRS patients were grown in culture, either differentiated or submerged. HSNECs were treated for 30 minutes with 0-50 microM of AC and were subsequently analyzed by real-time polymerase chain reaction and ELISA to determine IL-8 and human beta-defensin (HBD) 2 expression. Total glutathione was measured to see the oxidative stress within the treatment range. In primary HSNEC, IL-8 mRNA levels decreased dose dependently in the range of 10-50 microM of AC with an eightfold decrease at 50 microM. In addition, a 125-fold decrease at 50 microM for IL-8 protein was observed. HBD-2 mRNA decreased twofold and HBD-2 protein decreased fourfold at 50 microM of AC in primary HSNEC. However, differentiated HSNEC showed a marginal decrease in a dose...Continue Reading

References

Jul 1, 1986·Toxicology Letters·R L SherwoodC Aranyi
Apr 1, 1968·Chemical Reviews·R L Stedman
Jan 1, 1983·Toxicology and Applied Pharmacology·C L Astry, G J Jakab
Sep 14, 1999·The American Journal of Physiology·L LiA Holian
Aug 10, 2000·The Journal of Allergy and Clinical Immunology·Y OuyangB M Freed
Jan 9, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·N Lopez-SouzaJ H Widdicombe
Mar 30, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Bahman SaatianErnst Wm Spannhake
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martti LaanGary P Anderson
Nov 9, 2004·American Journal of Respiratory and Critical Care Medicine·Hitesh S DeshmukhGeorge D Leikauf
Dec 4, 2004·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Eli O MeltzerUNKNOWN American Rhinologic Society
Dec 22, 2004·Archives of Otolaryngology--head & Neck Surgery·Joseph VandermeerRobert P Schleimer
Jan 15, 2005·Antioxidants & Redox Signaling·Giuseppe ValacchiAlbert van der Vliet
Oct 8, 2005·The Journal of Allergy and Clinical Immunology·Cherie LambertBrian M Freed
May 2, 2006·Toxicology and Applied Pharmacology·Floriane AugerAnne-Catherine Dazy
Oct 18, 2006·Archives of Otolaryngology--head & Neck Surgery·Itzhak Brook
Feb 8, 2007·American Journal of Rhinology·Murray RamanathanAndrew P Lane
Apr 12, 2007·American Journal of Rhinology·Ming-Ying LanAn-Hang Yang

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Citations

Apr 8, 2009·Current Allergy and Asthma Reports·Andrew P Lane
Jan 22, 2011·PloS One·Natalia Goldstein-DaruechNoam A Cohen
Dec 6, 2014·BMJ Open Respiratory Research·Olga Lucia Moncayo-NietoA John Simpson
Jun 9, 2010·Otolaryngologic Clinics of North America·Eng H OoiPeter-John Wormald
Apr 5, 2016·BioMed Research International·Veronica TrombitasSilviu Albu
Apr 24, 2012·The Laryngoscope·Nathan S AlexanderBradford A Woodworth
Jun 15, 2012·International Forum of Allergy & Rhinology·Douglas D RehTimothy L Smith
Nov 26, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Elisabetta PaceMark Gjomarkaj
Nov 4, 2016·International Forum of Allergy & Rhinology·Anuj TharakanMurugappan Ramanathan
Feb 21, 2018·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Dallin N ChristensenEugene H Chang
Jan 30, 2010·American Journal of Rhinology & Allergy·Laili IraniAna Navas-Acien
Apr 30, 2009·American Journal of Rhinology & Allergy·Edwin TamashiroNoam A Cohen
Dec 6, 2014·Current Opinion in Allergy and Clinical Immunology·Jin-Young Min, Bruce K Tan
Aug 9, 2016·Journal of Occupational and Environmental Medicine·Dean P JonesYoung-Mi Go
Feb 23, 2012·Viruses·Alexander W TarrJonathan K Ball
Aug 13, 2020·Cancers·Francisco AguayoDaniela Nuñez-Acurio
Jul 28, 2010·Expert Review of Respiratory Medicine·Douglas D Reh, Ana Navas-Acien
Dec 5, 2009·American Journal of Rhinology & Allergy·Douglas D RehAna Navas-Acien
Nov 4, 2008·Current Opinion in Allergy and Clinical Immunology

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