The cioAB genes from Pseudomonas aeruginosa code for a novel cyanide-insensitive terminal oxidase related to the cytochrome bd quinol oxidases

Molecular Microbiology
L CunninghamH D Williams

Abstract

The structural genes for the cyanide-insensitive terminal oxidase (CIO) of Pseudomonas aeruginosa were sequenced. The locus comprised two open reading frames, cioA and cioB, coding for gene products of 488 and 335 amino acid residues with predicted molecular masses of 54241 and 37016 Da respectively. These genes were encoded by a 2.7 kb transcript and probably comprise an operon. Upstream of a major transcriptional start site is a -10 promoter region and, approximately at nucleotides -50 and +13, there are sequences homologous to the binding site of the transcriptional regulator Anr. The deduced amino acid sequences of CioA and CioB are homologous to the cytochrome bd quinol oxidases of Escherichia coli and Azotobacter vinelandii. However, no cytochrome d-like signals were found in wild-type P. aeruginosa strains. An atypical cytochrome d-like signal was seen under low-aeration growth conditions but only in strains in which the cioAB genes were present on a high-copy-number plasmid. The appearance of these cytochrome d-like signals was not paralleled by a concomitant increase in CIO activity. These data support the hypothesis that the CIO of P. aeruginosa does not contain haem d. This raises the possibility that there is a fami...Continue Reading

Citations

Mar 23, 2010·Applied Microbiology and Biotechnology·Toshiharu Yakushi, Kazunobu Matsushita
Jan 7, 2009·Journal of Biochemistry·Tatsushi Mogi, Hideto Miyoshi
May 7, 2009·Journal of Biochemistry·Tatsushi MogiKazunobu Matsushita
May 23, 2002·Antimicrobial Agents and Chemotherapy·Maria BellantoneLarry L Hench
Jun 19, 2007·Applied and Environmental Microbiology·Alberto QuesadaRafael Blasco
Jul 3, 2013·Applied and Environmental Microbiology·Dongru QiuJizhong Zhou
Aug 19, 2008·Journal of Bacteriology·Emanuela FrangipaniDieter Haas
Oct 10, 2001·Journal of Bacteriology·L A Gallagher, C Manoil
Sep 18, 2004·Journal of Bacteriology·Michael D BraidMartha M Howe
Mar 8, 2008·Bioscience, Biotechnology, and Biochemistry·Wichai SoempholHirohide Toyama
Feb 16, 2013·Nature Reviews. Microbiology·Rachel L Morris, Thomas M Schmidt
Apr 3, 2004·Letters in Applied Microbiology·J E A Zlosnik, H D Williams
Sep 25, 2012·Environmental Microbiology·Víctor M Luque-AlmagroRafael Blasco
Jul 29, 2010·Molecular Microbiology·Jeffrey W SchertzerMarvin Whiteley
Mar 25, 2011·Environmental Microbiology·Lei YangSøren Molin
Mar 7, 2014·Environmental Microbiology Reports·Emanuela FrangipaniDieter Haas
Nov 26, 2009·Environmental Microbiology·Takuro KawakamiHiroyuki Arai
Jul 16, 2011·Biochimica Et Biophysica Acta·Vitaliy B BorisovMichael I Verkhovsky
Nov 18, 2010·Microbiology·Kenta SakuraiYasuo Igarashi
Jul 6, 2004·Journal of the American College of Cardiology·Gruschen R VeldtmanCarole A Warnes
Feb 18, 2015·Genome Biology and Evolution·Mauro Degli EspostiEsperanza Martínez-Romero
Jul 8, 2014·Frontiers in Cellular and Infection Microbiology·Yuta OkkotsuMichael J Schurr
Aug 8, 2009·FEMS Microbiology Letters·Emanuela Frangipani, Dieter Haas

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