The circRNA-MYLK plays oncogenic roles in the Hep-2 cell line by sponging microRNA-145-5p

General Physiology and Biophysics
Yao ChenXiaoyan Sun

Abstract

For the exploration of circular RNA light chain kinase (circRNA-MYLK), siRNA#1 and siRNA#2 targeting circRNA-MYLK as well as microRNA(miR)-145-5p inhibitor were transfected. Viability was valued with the CCK-8. The protein expression was examined relying on Western blot. The expression of circRNA-MYLK or miR-145-5p was tested depending on qRT-PCR. The apoptotic/migration/invasion rate was separately measured by the Annexin v-FITC/PI with flow cytometer or chambers assays. CircRNA-MYLK was overexpressed in tumor tissue. Silencing circRNA-MYLK induced the inhibitions of viability, invasion and migration, as well as the blocks of MEK/ERK and NF-κB cascades, however, silencing circRNA-MYLK led to provoking of apoptosis. Besides, circRNA-MYLK silencing stimulated the over-production of miR-145-5p, whose silencing abolished the effects of siRNA#1 and siRNA#2 of circRNA-MYLK on those factors above. The circRNA-MYLK had oncogenic roles via targeting miR-145-5p in the Hep-2 cell line via stimulating MEK/ERK and NF-κB cascades.

Citations

Aug 26, 2020·Journal of Clinical Laboratory Analysis·Pan HeBin Sheng
May 11, 2021·Pathology, Research and Practice·Shanying WuMingfang Fan
Oct 8, 2021·Journal of Clinical Laboratory Analysis·Limin MiaoHua Yuan

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