The cis-expression of the coat protein of turnip mosaic virus is essential for viral intercellular movement in plants.

Molecular Plant Pathology
Zhaoji DaiAiming Wang

Abstract

To establish infection, plant viruses are evolutionarily empowered with the ability to spread intercellularly. Potyviruses represent the largest group of known plant-infecting RNA viruses, including many agriculturally important viruses. To better understand intercellular movement of potyviruses, we used turnip mosaic virus (TuMV) as a model and constructed a double-fluorescent (green and mCherry) protein-tagged TuMV infectious clone, which allows distinct observation of primary and secondary infected cells. We conducted a series of deletion and mutation analyses to characterize the role of TuMV coat protein (CP) in viral intercellular movement. TuMV CP has 288 amino acids and is composed of three domains: the N-terminus (amino acids 1-97), the core (amino acids 98-245), and the C-terminus (amino acids 246-288). We found that deletion of CP or its segments amino acids 51-199, amino acids 200-283, or amino acids 265-274 abolished the ability of TuMV to spread intercellularly but did not affect virus replication. Interestingly, deletion of amino acids 6-50 in the N-terminus domain resulted in the formation of aberrant virions but did not significantly compromise TuMV cell-to-cell and systemic movement. We identified the charged r...Continue Reading

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Citations

Apr 24, 2021·Annual Review of Phytopathology·Xiuling YangAiming Wang
Oct 10, 2021·Molecular Plant-microbe Interactions : MPMI

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Methods Mentioned

BETA
Confocal microscopy
transfection
transmission electron microscopy
reverse transcription PCR
Infection
transgenic
Confocal
PCR
Y2H

Software Mentioned

ImageJ

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