PMID: 2105293Feb 1, 1990Paper

The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a protein homologous to a site-specific recombinase

Journal of Bacteriology
T SatoY Kobayashi

Abstract

The nucleotide sequence of the sporulation gene spoIVC cisA in Bacillus subtilis was determined and found to encode a protein of 500 amino acid residues with a calculated molecular weight of 57,481, which is in good agreement with the size of the gene product estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The amino acid sequence of the N-terminal region of this protein is homologous to the site-specific DNA recombinases. Hybridization of a 3.6-kilobase EcoRI fragment carrying the spoIVC cisA gene with the EcoRI-restricted chromosomal DNA prepared from cells of various stages showed that DNA rearrangement occurs only in the mother cell in the region adjacent to spoIVC cisA 3 h after the initiation of sporulation. This result coincides with that of Stragier et al. (P. Stragier, B. Kunkel, L. Kroos, and R. Losick, Science 243:507-512, 1989). The timing of the DNA rearrangement coincides very well with the timing of spoIVC cisA gene expression. The DNA rearrangement was not observed in spoIVC cisA mutants. These results strongly suggest that the spoIVC cisA gene encodes a site-specific DNA recombinase having a very important role in sporulation.

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Citations

Nov 1, 1990·Molecular Microbiology·P Stragier, R Losick
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·H M Thorpe, M C Smith
Mar 20, 2010·Biochemical Society Transactions·Margaret C M SmithPaul A Rowley
Aug 29, 2013·Critical Reviews in Biochemistry and Molecular Biology·Gregory D Van Duyne, Karen Rutherford
Sep 11, 2013·Molecular Microbiology·Kimihiro AbeTsutomu Sato
Feb 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J W Gober
Jul 4, 2013·Nucleic Acids Research·Karen RutherfordGregory D Van Duyne
Apr 16, 2003·Molecular Microbiology·Jeralyn D Haraldsen, Abraham L Sonenshein
Feb 26, 2000·Annual Review of Genetics·T Komano
May 15, 2002·Cloning and Stem Cells·Andreas F Kolb
Oct 17, 2012·Applied Microbiology and Biotechnology·Tetsurou MuroiMitsuru Haruki
Aug 5, 2008·Molecular Microbiology·Teppei AbeAkira Muto
Dec 21, 2013·FEMS Microbiology Reviews·Anna KonovalovaLee Kroos
Jan 9, 2007·Journal of Bacteriology·Shigeo HosoyaTsutomu Sato
Aug 9, 2011·Applied Microbiology and Biotechnology·Nobutaka HiranoMitsuru Haruki
Aug 23, 2006·Journal of Bacteriology·Alisa W SerioAbraham L Sonenshein
Aug 10, 2001·Microbiology·A BreünerK Hammer
Jul 1, 1994·Journal of Virology·A YuE Haggård-Ljungquist
Mar 1, 1995·Microbiological Reviews·W G Haldenwang
Sep 10, 2015·Microbiology Spectrum·Margaret C M Smith

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