The citrus flavonone hesperetin attenuates the nuclear translocation of aryl hydrocarbon receptor

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Yan Qin TanLai K Leung

Abstract

The environmental polycyclic aromatic hydrocarbons (PAH) and dioxins are carcinogens and their adverse effects have been largely attributed to the activation of AhR. Hesperetin is a flavonone found abundantly in citrus fruits and has been shown to be a biologically active agent. In the present study, the effect of hesperetin on the nuclear translocation of AhR and the downstream gene expression was investigated in MCF-7 cells. Confocal microscopy indicated that 7, 12-dimethylbenz[α]anthracene (DMBA) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) -induced nuclear translocation of AhR was deterred by hesperetin treatment. The reduced nuclear translocation could also be observed in Western analysis. Reporter-gene assay further illustrated that the induced XRE transactivation was weakened by the treatment of hesperetin. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay demonstrated that the gene expressions of CYP1A1, 1A2, and 1B1 followed the same pattern of AhR translocation. These results suggested that hesperetin counteracted AhR transactivation and suppressed the downstream gene expression.

Citations

Aug 23, 2019·Critical Reviews in Toxicology·Tian YangYing-Yong Zhao
Jan 28, 2021·International Journal of Molecular Sciences·Monika SzelestTomasz Plech
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elizabeth Goya-JorgeRosa M Giner

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