The class V myosin motor, myosin 5c, localizes to mature secretory vesicles and facilitates exocytosis in lacrimal acini.

American Journal of Physiology. Cell Physiology
Ronald R MarchellettaSarah F Hamm-Alvarez

Abstract

We investigated the role of the actin-based myosin motor, myosin 5c (Myo5c) in vesicle transport in exocrine secretion. Lacrimal gland acinar cells (LGAC) are the major source for the regulated secretion of proteins from the lacrimal gland into the tear film. Confocal fluorescence and immunogold electron microscopy revealed that Myo5c was associated with secretory vesicles in primary rabbit LGAC. Upon stimulation of secretion with the muscarinic agonist, carbachol, Myo5c was also detected in association with actin-coated fusion intermediates. Adenovirus-mediated expression of green fluorescent protein (GFP) fused to the tail domain of Myo5c (Ad-GFP-Myo5c-tail) showed that this protein was localized to secretory vesicles. Furthermore, its expression induced a significant (P < or = 0.05) decrease in carbachol-stimulated release of two secretory vesicle content markers, secretory component and syncollin-GFP. Adenovirus-mediated expression of GFP appended to the full-length Myo5c (Ad-GFP-Myo5c-full) was used in parallel with adenovirus-mediated expression of GFP-Myo5c-tail in LGAC to compare various parameters of secretory vesicles labeled with either GFP-labeled protein in resting and stimulated LGAC. These studies revealed that t...Continue Reading

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Citations

Dec 6, 2011·Cytotechnology·Janette ContrerasSarah F Hamm-Alvarez
Jun 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Erez GeronBen-Zion Shilo
Sep 11, 2009·Molecular Biology of the Cell·Damon T JacobsRichard E Cheney
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Apr 29, 2011·American Journal of Physiology. Cell Physiology·Lilian ChiangSarah F Hamm-Alvarez
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Apr 15, 2016·American Journal of Physiology. Cell Physiology·Zhen MengSarah F Hamm-Alvarez
Oct 18, 2014·The Journal of Biological Chemistry·Jarred J BultemaSantiago M Di Pietro

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