The classical swine fever virus N-terminal protease N(pro) binds to cellular HAX-1.

The Journal of General Virology
H L JohnsJulian Seago

Abstract

The positive-stranded RNA genome of classical swine fever virus (CSFV) encodes 12 known proteins. The first protein to be translated is the N-terminal protease (N(pro)). N(pro) helps evade the innate interferon response by targeting interferon regulatory factor-3 for proteasomal degradation and also participates in the evasion of dsRNA-induced apoptosis. To elucidate the mechanisms by which N(pro) functions, we performed a yeast two-hybrid screen in which the anti-apoptotic protein HAX-1 was identified. The N(pro)-HAX-1 interaction was confirmed using co-precipitation assays. A dramatic redistribution of both N(pro) and HAX-1 was observed in co-transfected cells, as well as in transfected cells infected with wild-type CSFV, but not in cells infected with an N(pro)-deleted CSFV strain.

References

Mar 14, 1998·Journal of Virology·I RouillerT Wileman
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·A R GallagherR Witzgall
Sep 5, 2002·Veterinary Immunology and Immunopathology·Ana CarrilloJosé Yélamos
Sep 17, 2004·The Journal of Biological Chemistry·Lucia CilentiAntonis S Zervos
Jan 25, 2005·The Veterinary Quarterly·S RibbensA de Kruif
Oct 18, 2005·Journal of Virology·Venkat S R K YedavalliLi-Min Huang
Aug 19, 2006·Circulation Research·James Shaw, Lorrie A Kirshenbaum
Sep 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yasushi KawaguchiMasako Hara
Jan 24, 2007·Journal of Molecular Biology·Elizabeth VafiadakiAikaterini Kontrogianni-Konstantopoulos
Oct 12, 2007·Journal of Cellular Physiology·Suhasini Modem, Thipparthi R Reddy
May 13, 2008·Journal of Molecular Biology·Delphine M LeesJohn F Marshall
Jul 18, 2008·The Journal of General Virology·Virginie DoceulJulian Seago
Jun 16, 2009·Biochimica Et Biophysica Acta·Bengt Fadeel, Ewa Grzybowska
Oct 23, 2009·Veterinary Microbiology·Ernst Peterhans, Matthias Schweizer

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Citations

Aug 9, 2012·Biologicals : Journal of the International Association of Biological Standardization·Ernst Peterhans, Matthias Schweizer
Apr 14, 2015·Cellular & Molecular Immunology·Susanne WolkerstorferGertrude Achatz-Straussberger
Dec 17, 2014·Virus Research·Wei JiCheng-Qiang He
Mar 21, 2018·Archives of Virology·Wang DongYanming Zhang

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