The clinical significance of miR-335, miR-124, miR-218 and miR-484 downregulation in gastric cancer

Molecular Biology Reports
Ali ZareHamid Ghaedi

Abstract

Gastric cancer (GC) is one of the leading types of malignancy worldwide, particularly in Asian populations. Although the exact molecular mechanism of GC development remains unknown, microRNA (miRNA) has recently been shown to be involved. The current study aims to investigate the expression levels of bioinformatically ranked miRNAs in gastric tissues. Using bioinformatics tools, we prioritized miRNAs thought to be implicated in GC. Furthermore, polyA-qPCR was used to validate bioinformatics findings in 40 GC, 31 normal gastric tissue (NG) and 45 gastric dysplasia (GD) samples. As identified by bioinformatics analysis, miR-335 was shown to be the top-ranked miRNA implicated in GC. Moreover, a significant downregulation of miR-335, miR-124, miR-218 and miR-484 was found in GC and GD compared to NG samples. We found bioinformatics to be an efficient approach to finding candidate miRNAs relevant to GC development. Finally, the findings show that downregulation of miRNAs such as miR-124 and miR-218 in gastric tissue can be a significant indicator for neoplastic transformation.

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Citations

Aug 8, 2019·Cancer Medicine·Xinqi JiaShan Yu
Jan 3, 2020·The Kaohsiung Journal of Medical Sciences·Xiao-Qing DouShi-Qian Zhang
Feb 18, 2020·Cancer Biomarkers : Section a of Disease Markers·Weijia ZhangHai Zeng
Jan 8, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Jan 24, 2021·Cancer Cell International·Yi GaoJing Zhang
Nov 13, 2020·Clinical and Experimental Gastroenterology·Khadijeh Kalan FarmanfarmaHamid Salehiniya
May 29, 2021·Environmental Science and Pollution Research International·Marwa M MahmoudNadia M Hamdy
May 29, 2021·OncoTargets and Therapy·Lingling YeQuan An Zhang

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Methods Mentioned

BETA
RNA-Seq
scraping

Software Mentioned

Galaxy
SPSS
MiRTargetLinkTarget
Cancer RNA - Seq Nexus

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