The clinicopathological significance of monocarboxylate transporters in testicular germ cell tumors

Oncotarget
Eduardo C A SilvaCéline Pinheiro

Abstract

Metabolic reprogramming is one of the hallmarks of cancer. The hyperglycolytic phenotype is often associated with the overexpression of metabolism-associated proteins, such as monocarboxylate transporters (MCTs). MCTs are little explored in germ cell tumors (GCTs), thus, the opportunity to understand the relevance of these metabolic markers and their chaperone CD147 in this type of tumor arises. The main aim of this study was to evaluate the expression of MCT1, MCT2, MCT4 and CD147 in testicular GCT samples and the clinicopathological significance of these metabolism related proteins. MCT1, MCT4 and CD147 were associated with higher stages, higher M and N stages and histological type, while MCT4 was also associated with higher risk stratification, presence of vascular invasion, and lower overall and event free survival. MCT4 silencing in JEG-3 had no significant effect in cell viability, proliferation and death, as well as extracellular levels of glucose and lactate. However, MCT4-silenced cells showed an increase in migration and invasion. The proteins herein studied, with the exception of MCT2, were associated with characteristics of worse prognosis, lower global and event free survival of patients with GCTs. Also, in vitro M...Continue Reading

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Citations

Jul 19, 2019·Frontiers in Endocrinology·Murilo BonatelliCéline Pinheiro
Aug 10, 2019·Molecular Metabolism·Valéry L PayenPierre Sonveaux
Mar 7, 2020·Cancer Letters·Vera Miranda-GonçalvesCarmen Jerónimo
Jul 13, 2021·Frontiers in Oncology·Murilo BonatelliCéline Pinheiro

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Methods Mentioned

BETA
flow cytometry
genotyping
Protein Assay

Software Mentioned

SPSS
OpenCFU
BD Accuri C6
GraphPad Prism
GraphPad

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