The cloning and expression of a new guanylyl cyclase orphan receptor.

The Journal of Biological Chemistry
S SchulzD L Garbers

Abstract

A novel membrane form of guanylyl cyclase (GC-G) has been identified through the isolation of a full-length cDNA clone; it is predicted to contain an extracellular ligand binding domain, a single transmembrane segment, and intracellular protein kinase-like and cyclase catalytic domains. That GC-G represents a guanylyl cyclase was confirmed by both transient expression in COS-7 cells and stable expression in H293 cells. Endogenous cyclic GMP concentrations of transfected or stable cells, however, were much higher than control cells, suggesting an inability of the cells to effectively regulate GC-G cyclase activity. Of six Cys residues found within the extracellular domain of guanylyl cyclase-A (GC-A), the receptor for atrial natriuretic peptide, five are conserved within GC-G. Ligands for the other cyclase receptors, nevertheless, failed to stimulate GC-G expressed in transient or stable cells, suggesting that the unknown ligands possess a structure different from the natriuretic peptides or heat-stable enterotoxins. 125I-ANP also failed to bind to H293 cells overexpressing GC-G. Based on Northern hybridization, mRNA for GC-G was predominantly expressed in lung, intestine, and skeletal muscle. Using the candidate gene approach t...Continue Reading

References

Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M G CurrieC E Smith
Nov 1, 1992·The American Journal of Physiology·J G PorterA A Protter
Jan 1, 1991·Methods in Enzymology·S E DominoD L Garbers
Nov 30, 1990·Cell·S SchulzD L Garbers
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·H J FülleD L Garbers
May 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L R Forte, M G Currie
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·D G LoweJ B Hurley
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·R B YangH J Fülle
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F K HamraC E Smith
May 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L B RoweE H Birkenmeier
May 28, 1993·Biochemical and Biophysical Research Communications·M FuruyaH Matsuo
Dec 26, 1995·Biochemical and Biophysical Research Communications·M KojimaK Kangawa
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·I KishimotoD L Garbers
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·S YuD L Garbers

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Jun 14, 2005·Pflügers Archiv : European journal of physiology·Aleksandra Sindić, Eberhard Schlatter
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Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·B J Wedel, D L Garbers
Jul 17, 1999·European Journal of Biochemistry·M HasegawaY Shimonishi
Oct 16, 2009·Human Reproduction·Ruey-Bing YangYen-Hua Huang
Jun 9, 2000·Annual Review of Neuroscience·A D Gibson, D L Garbers
Jan 18, 2008·Journal of the American Society of Nephrology : JASN·Heng LinRuey-Bing Yang
Jan 1, 2013·ISRN Nephrology·Aleksandra Sindic
Feb 4, 2016·Journal of the American Society of Nephrology : JASN·Yuehan ZhouWalter F Boron
Feb 12, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·M Hasegawa, Y Shimonishi
Jul 1, 2009·The Journal of Comparative Neurology·Cambrian Y LiuDavid S Koos
Mar 15, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kaori IioNorio Suzuki
Jan 24, 2006·General and Comparative Endocrinology·Junying ZhengR Douglas Watson
Mar 1, 2006·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Koji Inoue, Yoshio Takei
May 25, 2005·Peptides·Stephanie Schulz
Jan 11, 2002·Developmental Biology·C P RobertsonH Roelink
Sep 15, 2011·Cellular Signalling·Lincoln R Potter
May 13, 1999·Biochimica Et Biophysica Acta·J W Denninger, M A Marletta
Mar 29, 2014·Frontiers in Molecular Neuroscience·Teresa DudaRameshwar K Sharma
Oct 4, 2016·Frontiers in Molecular Neuroscience·Rameshwar K SharmaClint L Makino
Jun 8, 2004·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Hui-Fen LiuChi-Ying Lee
Sep 13, 2007·American Journal of Physiology. Endocrinology and Metabolism·Silvana A AndricTatjana S Kostic
Oct 18, 2013·American Journal of Physiology. Renal Physiology·Lara A Skelton, Walter F Boron
Nov 21, 2019·Biological Chemistry·Franz Hofmann
Apr 1, 2016·Physiological Reviews·Michaela Kuhn
Nov 27, 2009·Molecular and Cellular Biochemistry·Teresa Duda
Nov 17, 2009·Molecular and Cellular Biochemistry·Anna Jankowska, Jerzy B Warchol
Dec 3, 1999·Methods : a Companion to Methods in Enzymology·D L Garbers

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