PMID: 8596675Oct 1, 1995Paper

The cloning, expression and sequence analysis of a second Porphyromonas gingivalis gene that codes for a protein involved in hemagglutination

Oral Microbiology and Immunology
A Progulske-FoxJ A Banas

Abstract

It has been suggested that Porphyromonas gingivalis may possess more than one hemagglutinin. We have previously reported the cloning of a gene (hagA) that encodes a hemagglutinin. In this study we report the cloning, characterization, and sequencing of a second gene (hagB) that encodes a protein that also appears to be involved in hemagglutination. Antiserum to the clone (ST 7) was found to inhibit hemagglutination by P. gingivalis 381, and hemagglutinating inhibition activity of anti-P. gingivalis antiserum was reduced by adsorption of the antiserum with cells of clone ST 7. Restriction mapping and Southern analysis indicates there is little or no DNA homology between this cloned 4.8-kb HindIII DNA fragment and a cloned hemagglutinin gene we have previously described. Minicell analysis of the cloned P. gingivalis chromosomal DNA fragment revealed that the major gene product is a 49-kDa protein. Immunoaffinity chromatography using purified rabbit immunoglobulin G against the cloned protein resulted in the purification of a major reactive 49- to 50-kDa protein from a P. gingivalis cell lysate. Nucleotide sequence analysis revealed the hagB open reading frame to be 1053 nucleotides in length with a mol% G+C of 59.9% coding for a ...Continue Reading

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Citations

Jan 12, 2001·Oral Microbiology and Immunology·R J Lamont, H F Jenkinson
Apr 1, 1996·Oral Microbiology and Immunology·G Lépine, A Progulske-Fox
Feb 1, 1997·Oral Microbiology and Immunology·A A DeCarlo, G J Harber
Oct 16, 1999·Periodontology 2000·S C HoltC A Genco
Oct 6, 2010·BMC Microbiology·Jorg BrunnerWim Crielaard
Feb 21, 2003·Journal of Periodontology·Kouji KaizukaYoshimitsu Abiko
Dec 7, 2007·Microbial Pathogenesis·Koichi HiratsukaYoshimitsu Abiko
Apr 27, 2004·Periodontology 2000·Neil M O'Brien-SimpsonEric C Reynolds
May 12, 2001·European Journal of Oral Sciences·Y HosogiY Abiko
Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A CurtisM Rangarajan
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Y Abiko
Apr 1, 1997·Advances in Dental Research·R P EllenP M Nghiem
Apr 19, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dalia E GaddisJannet Katz
Sep 24, 1998·Infection and Immunity·E KozarovA Progulske-Fox

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